公开(公告)号：US20240125729A1

公开(公告)日：2024-04-18

申请号：US18277258

申请日：2022-02-18

申请人： THE JOHNS HOPKINS UNIVERSITY

发明人： Netzahualcoyotl ARROYO ,  Miguel Aller PELLITERO ,  Jonathan D. MAHLUM ,  Samuel D. CURTIS

IPC分类号： G01N27/327 ,  A61B5/00 ,  A61B5/1477 ,  G01N27/48 ,  G01N33/543

CPC分类号： G01N27/3277 ,  A61B5/1477 ,  A61B5/685 ,  G01N27/3276 ,  G01N27/48 ,  G01N33/5438

摘要： Provided herein are methods of detecting target molecules using electrochemical sensors that comprise biomolecular receptor-bound redox reporters. Related systems and computer readable media are also provided.

公开(公告)号：US11867704B2

公开(公告)日：2024-01-09

申请号：US16756029

申请日：2018-10-15

申请人： UNIVERSITEIT ANTWERPEN

发明人： Karolien De Wael ,  Mats De Jong ,  Anca Stefana Florea ,  Nick Sleegers

IPC分类号： G01N27/48 ,  G01N33/94 ,  G01N27/327 ,  G01N27/416 ,  G01N33/487

CPC分类号： G01N33/94 ,  G01N27/327 ,  G01N27/4161 ,  G01N27/48 ,  G01N33/48714 ,  G01N33/946 ,  Y10S436/901

摘要： A method for determining a narcotic in a mixture comprising at least one interferant. The method comprises: (a) determining a voltage at which, in absence of the interferant, a voltammetric signal of the narcotic can be detected; (b) contacting an electrode with the mixture comprising the at least one interferant and potentially comprising the narcotic; (c) applying a pretreatment potential to the electrode for a duration of at least 5 seconds, the pretreatment potential measuring between −0.4 V and −2 V versus Ag/AgCl; (d) measuring a voltammetric response of the mixture, the measurement comprising at least the determined voltage; and (e) determining whether the narcotic is present in the mixture by analyzing whether the voltammetric signal of the narcotic, resolved from a voltammetric signal of the interferant, can be detected in the measured voltammetric response.

公开(公告)号：US20230400430A1

公开(公告)日：2023-12-14

申请号：US18302588

申请日：2023-04-18

申请人： KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

发明人： Yi Jae LEE ,  Seung Hyeon KO

IPC分类号： G01N27/327 ,  G01N27/48 ,  C25D13/02 ,  C25D13/12 ,  A61B5/1468

CPC分类号： G01N27/3275 ,  G01N27/48 ,  C25D13/02 ,  C25D13/12 ,  A61B5/1468 ,  A61B2562/125

摘要： The present disclosure relates to an electrochemical sensor for simultaneous detection of dopamine and serotonin including an electrode containing a reduced graphene oxide (rGO), poly(3,4-ethylenedioxythiophene):poly(4-styrenesulfonate) (PEDOT:PSS), and Nafion, wherein the sensor has high interfacial conductivity and electrocatalytic properties and further improves the negatively charged electrode interface, thereby enabling high sensitivity selective measurement of dopamine and serotonin. In addition, since the sensor according to the present disclosure is stable for a long time and has high reproducibility, it can be used for clinical diagnosis of various brain and neurological diseases, drug treatment, biological research using changes in the concentration of neurotransmitters, and biochip application fields.

公开(公告)号：US11834697B2

公开(公告)日：2023-12-05

申请号：US16644539

申请日：2018-09-17

申请人： OXFORD UNIVERSITY INNOVATION LIMITED

发明人： Richard Guy Compton ,  Sabine Kuss

IPC分类号： C12Q1/26 ,  C12Q1/02 ,  G01N27/327 ,  G01N27/416 ,  G01N27/48

CPC分类号： C12Q1/26 ,  C12Q1/025 ,  G01N27/3277 ,  G01N27/4161 ,  G01N27/48 ,  G01N2333/90216

摘要： A method of determining the presence of bacteria expressing cytochrome c oxidase (‘the bacteria’), the method comprising: —providing a sample suspected of containing the bacteria; —providing a compound that has two redox states: a reduced state and an oxidised state, wherein cytochrome c oxidase can convert the compound from its reduced state to its oxidised state; —contacting an electrode either with (i) the compound in its oxidised state in the presence of the sample, then applying a reductive potential and measuring the current at the electrode; or (ii) the compound in its reduced state in the presence of the sample, then applying an oxidative potential and measuring the current at the electrode; and—comparing the magnitude of the current produced by the reductive potential or oxidative potential in the presence of the sample suspected of containing the bacteria with the magnitude of the current produced under the same conditions, but in the absence of the sample suspected of containing the bacteria, wherein a difference between the magnitude of current produced in the presence of the sample suspected of containing the bacteria and the magnitude of current produced in the absence of the sample suspected of containing the bacteria indicates the presence of the bacteria. Also provided herein is a sensor for determining the presence of bacteria expressing cytochrome c oxidase.

公开(公告)号：US20230125201A1

公开(公告)日：2023-04-27

申请号：US17973143

申请日：2022-10-25

申请人： Emanuela Silvana Andreescu ,  Abd Ur Rehman ,  Daniel Andreescu

发明人： Emanuela Silvana Andreescu ,  Abd Ur Rehman ,  Daniel Andreescu

IPC分类号： G01N21/78 ,  G01N27/48

摘要： A method and sensing system for the determination per and poly-fluoroalkyl substances (PFASs) is disclosed, wherein the probe is based on measurement of the redox activity of a redox indicator. The method includes adding a PFAS compound to an indicator solution, gel, 3D printed object, electrode or a sensing surface containing and measuring the change in the indicator signal as a function of PFAS concentration. Further provided is a portable sensor for rapid monitoring of the presence and PFAS concentrations. The present invention includes deposition of the indicator component within a method, assay, apparatus and sensing platform. Further provided is a composite electrode and sensor with binding and signaling activity for a broad range of PFAS, as well as printing ink compositions that incorporate the redox indicator.

公开(公告)号：US20230087763A1

公开(公告)日：2023-03-23

申请号：US17796953

申请日：2021-02-03

申请人： CARDIOVASCULAR LAB S.P.A.

发明人： Vincenzo Mazzaracchio ,  Neda Bagheri ,  Stefano Cinti ,  Danila Moscone ,  Fabiana Arduini

IPC分类号： G01N27/327 ,  G01N33/487 ,  G01N27/48 ,  G01N33/49 ,  G01N33/493 ,  G01N33/543

摘要： The present invention relates to a method for functionalising a cellulose support with metal nanoparticles comprising the steps of: depositing on the cellulose support a single aqueous solution containing the metal precursor in the form of acid or salt in a concentration from 1 to 6 mM; and placing the cellulose support at a temperature from 65° C. to 80° C. for a time from 10 to 40 minutes. The present invention also relates to a method for producing an electroanalytical sensor comprising said functionalised cellulose support with electrocatalytic and concentration properties of the metal marker at the working electrode and to a method for producing the electroanalytical sensor.

公开(公告)号：US20230074766A1

公开(公告)日：2023-03-09

申请号：US17949554

申请日：2022-09-21

申请人： Diagonal Bio AB

发明人： Kushagr Punyani ,  Per Andreas Nyberg ,  Sindre Sopstad ,  Martin Peacock ,  Linhongjia Xiong ,  Jae Yen Shin

IPC分类号： C12Q1/6844 ,  G01N21/80 ,  C12Q1/6825 ,  G01N27/416 ,  G01N27/48

摘要： Disclosed herein is a more sensitive and accurate method of monitoring the pH of a solution, wherein the pH of the solution is quantified as a function of the electrochemical response of the solution in a two or three-electrode electrochemical cell, wherein the solution comprises a compound capable of undergoing a change in its oxidation state and/or structural conformation as a function of the pH of the solution. Also disclosed are highly accelerated methods and processes enabling analysis of specific polynucleotide sequences in a sample, e.g. a biological sample. The methods disclosed herein are, for example, useful for rapid screening of a large amount of samples in a point-of-care setting.

公开(公告)号：US20230016698A1

公开(公告)日：2023-01-19

申请号：US17895152

申请日：2022-08-25

申请人： KING FAHD UNIVERSITY OF PETROLEUM AND MINERALS

发明人： Abdel-Naser M. KAWDE ,  Nurudeen Adewale ODENWUNMI

IPC分类号： G01N27/327 ,  G01N27/30 ,  G01N33/66 ,  G01N33/68 ,  G01N27/48

摘要： A method of using a graphite electrode to measure a concentration of glucose or methionine from a biological sample is described. A mechanical pencil lead may be used as the graphite electrode, and the biological sample may come from a patient's serum. The glucose or methionine may produce a peak current response within a range of 0.4-0.8 V when the sample is subjected to linear scan voltammetry.

公开(公告)号：US11518689B2

公开(公告)日：2022-12-06

申请号：US16869471

申请日：2020-05-07

申请人： Battelle Memorial Institute

发明人： Radha K. Motkuri ,  Sayandev Chatterjee ,  Dushyant Barpaga ,  Bernard P. McGrail

IPC分类号： C02F1/28 ,  B01J31/24 ,  B01J27/198 ,  B01J27/188 ,  B01J27/19 ,  B01J27/195 ,  B01J35/00 ,  A62D3/115 ,  A62D3/40 ,  A62D3/38 ,  C02F1/72 ,  C02F1/30 ,  G01N27/48 ,  B01J20/22 ,  C02F1/32 ,  C02F1/58 ,  G01N27/07 ,  G01N27/22 ,  G01N33/18 ,  A62D101/22 ,  C02F101/36 ,  C02F103/06

摘要： Materials for binding per- and polyfluoroalkyl substances (PFAS) are disclosed. A fluidic device comprising the materials for detection and quantification of PFAS in a sample is disclosed. The fluidic device may be configured for multiplexed analyses. Also disclosed are methods for sorbing and remediating PFAS in a sample. The sample may be groundwater containing, or suspected of containing, one or more PFAS.

公开(公告)号：US20220326172A1

公开(公告)日：2022-10-13

申请号：US17638370

申请日：2020-08-31

申请人： UNIVERSITEIT ANTWERPEN

发明人： Karolien DE WAEL ,  Saranya THIRUVOTTRIYUR SHANMUGAM

IPC分类号： G01N27/30 ,  G01N27/48 ,  G01N27/49

摘要： A source of formaldehyde for methylating a primary or secondary amine is part of an electrochemical measurement. The source of formaldehyde may be an adduct of formaldehyde.