公开(公告)号：US20230101196A1

公开(公告)日：2023-03-30

申请号：US16969588

申请日：2019-12-31

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong CAO ,  Ningtao ZHOU ,  Li ZHOU ,  Qin ZHU ,  Jinglin HE ,  Zhongliang XIAO ,  Yumin HE ,  Zemeng FENG ,  Yulong YIN

IPC: G01N27/327 ,  G01N27/48 ,  C07K1/107

Abstract: A method and a sensor for detecting L-arginine are provided. The method includes synthesizing ferrocene-functionalized hexadecapeptide dithiocyclopentane (FC-P16 Peptide), preparing a polypeptide composite membrane-modified electrode (FC-P16 Peptide/AuE), detecting L-Arg and other steps. The results show that the polypeptide composite membrane-modified electrode (FC-P16 Peptide/AuE) exhibits excellent electrochemical response properties to L-Arg. In 10 mmol/L phosphate-buffered saline (PBS, pH=7.4), the DPV response peak current of the polypeptide composite membrane-modified electrode has an excellent linear relationship with the L-Arg concentration of 1.0×10−13 mol/L to 1.0×10−7 mol/L, with a detection limit of 1.0×10−13 mol/L. With prominent reproducibility, repeatability and selectivity, the modified electrode has potential application in life science and nutritional health.

公开(公告)号：US20210025846A1

公开(公告)日：2021-01-28

申请号：US16955797

申请日：2019-08-24

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong CAO ,  Yuyu PENG ,  Chen LIU ,  Ling TANG ,  Qin ZHU ,  Min JIN ,  Jinglin HE ,  Zhongliang XIAO

IPC: G01N27/414

Abstract: A method and a sensor for detecting L-cysteine based on a 3,3′-dithiobis (1-propanesulfonate)-mercury composite membrane are provided. The method includes: implanting a p-well and an N-type substrate on field effect transistor substrate, constructing a source electrode and a drain electrode at the p-well, constructing a silicon dioxide layer on the substrate, plating an aluminum-copper alloy layer, a chromium-palladium alloy layer, and a gold film layer on a polysilicon gate substrate, constructing a silicon nitride layer on the substrate and the silicon dioxide layer; extending a gate part; preparing an ethanol solution of disodium 3,3′-dithiobis (1-propanesulfonate), cleaning and soaking gate gold electrode in the solution, followed by soaking in an ethanol solution of mercuric nitrate, and then washing the gate gold electrode; connecting electrode interfaces, inserting the electrode into PBS, and connecting power interfaces to a stabilized voltage supply, connecting a signal output interface to a multimeter.