公开(公告)号：US20180363008A1

公开(公告)日：2018-12-20

申请号：US16017157

申请日：2018-06-25

Applicant: CHILDREN'S MEDICAL CENTER CORPORATION

Inventor： Yi Zhang ,  Shogo Matoba

IPC: C12N15/877 ,  A01K67/027 ,  C12N15/113 ,  C12N5/0735 ,  C12N5/073

CPC classification number: C12N15/877 ,  A01K67/0273 ,  A01K2227/105 ,  C12N5/0604 ,  C12N5/0606 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2517/04 ,  C12Y201/01043

Abstract: The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and/or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and/or Suv39h2.

公开(公告)号：US20210155959A1

公开(公告)日：2021-05-27

申请号：US17045412

申请日：2019-04-05

Applicant: Children's Medical Center Corporation

Inventor： Yi Zhang ,  Shogo Matoba

IPC: C12N15/877 ,  C12N5/075 ,  C12N5/16 ,  C12N9/02 ,  A01K67/027

Abstract: The invention provides methods for improving cloning efficiency and modulating an imprinting control region. In particular embodiments, the invention provides methods for activating a repressed allele within an imprinting control region, thereby treating an imprinting associated disorder. In other embodiments, the invention provides methods for improving somatic cell nuclear transfer efficiency that involve Kdm4d overexpression is a Xist knockout donor cell.

公开(公告)号：US10266848B2

公开(公告)日：2019-04-23

申请号：US16017157

申请日：2018-06-25

Applicant: CHILDREN'S MEDICAL CENTER CORPORATION

Inventor： Yi Zhang ,  Shogo Matoba

IPC: C12N5/073 ,  A01K67/027 ,  C12N15/113 ,  C12N15/877 ,  C12N5/0735

Abstract: The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and/or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and/or Suv39h2.