公开(公告)号：US20240254452A1

公开(公告)日：2024-08-01

申请号：US18635153

申请日：2024-04-15

申请人： Whitehead Institute for Biomedical Research

发明人： Rudolf Jaenisch ,  Konrad Hoochedlinger

IPC分类号： C12N5/074 ,  A01K67/0273 ,  A01K67/0275 ,  C07K14/47 ,  C12N15/85 ,  C12N15/877

CPC分类号： C12N5/0696 ,  C12N15/85 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  C07K14/4702 ,  C12N15/8509 ,  C12N15/8775 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2830/003 ,  C12N2830/006

摘要： The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state.

公开(公告)号：US20180094240A1

公开(公告)日：2018-04-05

申请号：US15726672

申请日：2017-10-06

申请人： Kiho Lee ,  Randall S. Prather

发明人： Kiho Lee ,  Randall S. Prather

IPC分类号： C12N5/075 ,  C12N15/877 ,  A01K67/027

CPC分类号： C12N5/0609 ,  A01K67/0273 ,  A01K2227/108 ,  C12N15/877 ,  C12N15/8778 ,  C12N2500/14 ,  C12N2500/22 ,  C12N2501/405 ,  C12N2517/04 ,  C12N2517/10

摘要： The present invention provides novel methods for improving the efficiency of artificial activation of unfertilized mammalian oocytes by reducing the intracellular concentration of Zn2+ in the oocyte. The methods of the invention may additionally comprise a preceding step of increasing the intracellular concentration of Ca2+ in the oocyte prior to reduction of the intracellular Zn2+ concentration. The invention further provides unfertilized oocytes activated by the disclosed methods and viable mammalian animals produced from unfertilized oocytes activated by the disclosed methods.

公开(公告)号：US20170342386A1

公开(公告)日：2017-11-30

申请号：US15607028

申请日：2017-05-26

申请人： Whitehead Institute for Biomedical Research

发明人： Rudolf Jaenisch ,  Yaqub Hanna ,  Marius Wernig ,  Christopher J. Lengner ,  Alexander Meissner ,  Oliver Tobias Brambrink ,  G. Grant Welstead ,  Ruth Foreman

IPC分类号： C12N5/074 ,  C07K14/47 ,  G01N33/50 ,  C12N15/877 ,  C12N15/85 ,  A01K67/027

CPC分类号： C12N5/0696 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  C07K14/4702 ,  C12N15/8509 ,  C12N15/8775 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2506/11 ,  C12N2506/13 ,  C12N2800/30 ,  C12N2830/003 ,  C12N2830/006 ,  G01N33/5023 ,  G01N2333/91011

摘要： The disclosure relates to a method of reprogramming one or more somatic cells, e.g., partially differentiated or fully/terminally differentiated somatic cells, to a less differentiated state, e.g., a pluripotent or multipotent state. In further embodiments the invention also relates to reprogrammed somatic cells produced by methods of the invention, to uses of said cells, and to methods for identifying agents useful for reprogramming somatic cells.

公开(公告)号：US20170327846A1

公开(公告)日：2017-11-16

申请号：US15511348

申请日：2015-09-15

申请人： CHILDREN'S MEDICAL CENTER CORPORATION

发明人： Yi ZHANG ,  Shogo MATOBA

IPC分类号： C12N15/877 ,  A01K67/027 ,  C12N5/0735 ,  C12N15/113 ,  C12N5/073

CPC分类号： C12N15/877 ,  A01K67/0273 ,  A01K2227/105 ,  C12N5/0604 ,  C12N5/0606 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2517/04 ,  C12Y201/01043

摘要： The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and/or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and/or Suv39h2.

公开(公告)号：US09670464B2

公开(公告)日：2017-06-06

申请号：US14923321

申请日：2015-10-26

申请人： Whitehead Institute for Biomedical Research

发明人： Rudolf Jaenisch ,  Konrad Hochedlinger

IPC分类号： C12N5/00 ,  C12N5/074 ,  A01K67/027 ,  C07K14/47 ,  C12N15/85 ,  C12N15/877

CPC分类号： C12N5/0696 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  C07K14/4702 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/8775 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2830/003 ,  C12N2830/006

摘要： The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state.

公开(公告)号：US20150353885A1

公开(公告)日：2015-12-10

申请号：US14769350

申请日：2014-02-21

申请人： CELLECTIS

发明人： David Sourdive

IPC分类号： C12N5/071

CPC分类号： C12N5/0608 ,  A01H1/02 ,  A01H1/04 ,  A01K67/027 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/15 ,  A01K2217/30 ,  C12N15/8209 ,  C12N15/8213 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8287 ,  C12N15/907 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2800/40 ,  C12N2800/80 ,  C12N2999/007

摘要： The present invention relates to the field of genetic selection, where particular genetic traits or loci combinations are sought in a progeny resulting from genetic breeding. The invention provides genetic engineering solutions to select or counter-select the occurrence of genetic events.

摘要翻译： 本发明涉及遗传选择领域，其中遗传特性或基因座组合在遗传育种产生的后代中被寻求。 本发明提供了选择或反选遗传事件发生的遗传工程解决方案。

公开(公告)号：US20150247197A1

公开(公告)日：2015-09-03

申请号：US14436664

申请日：2013-10-18

申请人： TRANS OVA GENETICS

发明人： Poothappillai K. Kasinathan ,  Hong Wei ,  Mark F. Allan ,  David C. Faber

IPC分类号： C12Q1/68 ,  A01K67/027

CPC分类号： C12Q1/6883 ,  A01K67/02 ,  A01K67/0273 ,  A01K2207/00 ,  A01K2227/101 ,  A01K2267/02 ,  C12Q2600/124 ,  C12Q2600/156 ,  C12Q2600/158

摘要： Genetic tests, such as whole genome analysis (WGA), have been employed to identify genetically superior embryos. The disclosed methods extend in vitro culture time of embryos while awaiting results of genetic tests being performed on a portion of the same embryos. The disclosed methods also help expand the number of cells in each embryo before implantation in the recipient.

摘要翻译： 基因检测，如全基因组分析（WGA）已被用于鉴定遗传优良的胚胎。 所公开的方法延长了胚胎的体外培养时间，同时等待在同一胚胎的一部分上进行遗传测试的结果。 所公开的方法还有助于在植入接受者之前扩大每个胚胎中的细胞数量。

公开(公告)号：US20140359796A1

公开(公告)日：2014-12-04

申请号：US14263408

申请日：2014-04-28

申请人： Recombinetics, Inc.

发明人： Scott C. Fahrenkrug ,  Daniel F. Carlson

IPC分类号： A01K67/027 ,  C12N15/90

CPC分类号： A01K67/0276 ,  A01K67/0273 ,  A01K2217/077 ,  A01K2227/101 ,  A01K2227/103 ,  A01K2227/108 ,  A01K2267/02 ,  C12N15/8509 ,  C12N2800/30 ,  C12N2800/80

摘要： A genetically modified livestock animal, and methods of making and using the same, the animal comprising a genetic modification to disrupt a target gene selectively involved in gametogenesis, wherein the disruption of the target gene prevents formation of functional gametes of the animal. Animals that create progeny with donor genetics, and methods of making and using the same. Cells, and methods of making and using the cells, with a genetic modification to disrupt a target gene selectively involved in gametogenesis.

摘要翻译： 遗传修饰的家畜动物及其制备和使用方法，所述动物包含遗传修饰以破坏选择性参与配子形成的靶基因，其中靶基因的破坏阻止了动物的功能配子的形成。 用供体遗传学创造子代的动物，以及制作和使用它们的方法。 细胞以及制备和使用细胞的方法，具有遗传修饰以破坏选择性参与配子形成的靶基因。

公开(公告)号：US08785718B2

公开(公告)日：2014-07-22

申请号：US13645788

申请日：2012-10-05

申请人： Recombinetics, Inc.

发明人： Scott C. Fahrenkrug ,  Daniel F. Carlson ,  Aron M. Geurts

IPC分类号： C12N15/00 ,  A01K67/027 ,  A01K67/033 ,  C12N15/85

CPC分类号： C12N15/8509 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/058 ,  A01K2217/203 ,  A01K2227/101 ,  A01K2227/103 ,  A01K2227/105 ,  A01K2227/108 ,  A01K2267/03 ,  A01K2267/035 ,  A61D19/00 ,  C07K14/4712 ,  C12N15/1138 ,  C12N2015/8527 ,  C12N2310/14 ,  C12N2800/30 ,  C12N2800/90

摘要： Methods of using hypermethylated transposons to create genetically modified animals that express interfering RNAs are described.

摘要翻译： 描述了使用超甲基化转座子来产生表达干扰RNA的遗传修饰动物的方法。

公开(公告)号：US08309791B2

公开(公告)日：2012-11-13

申请号：US12504364

申请日：2009-07-16

申请人： Scott C. Fahrenkrug ,  Daniel F. Carlson ,  Aron M. Geurts

发明人： Scott C. Fahrenkrug ,  Daniel F. Carlson ,  Aron M. Geurts

IPC分类号： A01K67/027 ,  C12N15/00

CPC分类号： C12N15/8509 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/058 ,  A01K2217/203 ,  A01K2227/101 ,  A01K2227/103 ,  A01K2227/105 ,  A01K2227/108 ,  A01K2267/03 ,  A01K2267/035 ,  A61D19/00 ,  C07K14/4712 ,  C12N15/1138 ,  C12N2015/8527 ,  C12N2310/14 ,  C12N2800/30 ,  C12N2800/90

摘要： Transgenic artiodactyls are described as well as methods of making and using such artiodactyls.

摘要翻译： 本发明描述了转基因偶蹄菌，以及制备和使用这些偶氮酰基的方法。