公开(公告)号：US11612149B2

公开(公告)日：2023-03-28

申请号：US15743248

申请日：2016-07-08

申请人： CHUGAI SEIYAKU KABUSHIKI KAISHA

发明人： Koichi Jishage ,  Otoya Ueda ,  Naoko Wada ,  Takahiro Ishiguro ,  Yasuko Kinoshita

IPC分类号： A01K67/027 ,  C07K16/28 ,  C07K14/725 ,  C12N15/85 ,  C12N15/09 ,  G01N33/50 ,  G01N33/15

摘要： The present invention provides genetically modified non-human animals which are deficient in at least one or more types of CD3 genes selected from the group consisting of endogenous CD3ε, CD3δ, and CD3γ in its genome and functionally express at least one or more types of human CD3 genes selected from the group consisting of human CD3ε, CD3δ, and CD3γ. In the genetically modified non-human animals of the present invention, mature T cell differentiation and production can take place, and immunocompetent cells including T cells can exert their functions. The genetically modified non-human animals of the present invention enable efficient evaluation and screening in the development of therapeutic agents and therapeutic methods that use human CD3-mediated targeted drugs.

公开(公告)号：US10822420B2

公开(公告)日：2020-11-03

申请号：US14427355

申请日：2013-09-13

申请人： CHUGAI SEIYAKU KABUSHIKI KAISHA

发明人： Koichi Jishage ,  Otoya Ueda

IPC分类号： A61K49/00 ,  G01N33/68 ,  C07K16/28 ,  A01K67/027 ,  G01N33/50 ,  C07K14/715

摘要： The present invention provides a non-human animal in which a DNA comprising an hp7 sequence-encoding DNA and a poly A addition signal-encoding DNA added on the 3′ side of a DNA encoding an arbitrary foreign gene is inserted in the same reading frame as that of an arbitrary target gene present on the genome of the non-human animal.

公开(公告)号：US20230000063A1

公开(公告)日：2023-01-05

申请号：US17784735

申请日：2020-12-11

申请人： Chugai Seiyaku Kabushiki Kaisha

发明人： Manabu Wada ,  Naoko Wada ,  Otoya Ueda

IPC分类号： A01K67/027 ,  C12N15/62 ,  C12N5/10 ,  C12N9/02 ,  G01N33/15 ,  G01N33/50

摘要： An object of the present invention is to provide an evaluation system capable of detecting an extracellular purinergic receptor ligand minimally invasively, chronologically and systemically, and the present invention provides a genetically modified non-human animal expressing a first fusion protein and a second fusion protein for detecting an extracellular purinergic receptor ligand, in which the first fusion protein comprises a membrane protein that binds to a purinergic receptor ligand, and a first reporter protein, and the second fusion protein comprises a protein that binds to the membrane protein bound to the ligand, and a second reporter protein; and a cell thereof.

公开(公告)号：US20150225481A1

公开(公告)日：2015-08-13

申请号：US14427355

申请日：2013-09-13

申请人： CHUGAI SEIYAKU KABUSHIKI KAISHA

发明人： Koichi Jishage ,  Otoya Ueda

IPC分类号： C07K16/28 ,  A61K49/00 ,  G01N33/68 ,  A01K67/027

摘要： The present invention provides a non-human animal in which a DNA comprising an hp7 sequence-encoding DNA and a poly A addition signal-encoding DNA added on the 3′ side of a DNA encoding an arbitrary foreign gene is inserted in the same reading frame as that of an arbitrary target gene present on the genome of the non-human animal.

摘要翻译： 本发明提供一种非人动物，其中将包含编码hp7序列的DNA和编码任意外来基因的DNA的3'侧加入的加聚信号编码DNA的DNA插入同一读码框中 作为存在于非人动物基因组上的任意靶基因。