公开(公告)号：US10087249B2

公开(公告)日：2018-10-02

申请号：US15068178

申请日：2016-03-11

申请人： COMPLIX SA

发明人： Johan Desmet ,  Ignace Lasters ,  Maria Henderikx ,  Anita Wehnert

IPC分类号： C40B40/10 ,  C07K16/24 ,  C07K14/00 ,  C12N15/10 ,  C07K16/00 ,  C40B50/12

摘要： The invention provides single-chain Alphabody library comprising at least 100 different-sequence single-chain Alphabody polypeptides, wherein said Alphabody polypeptides differ from each other in at least one of a defined set of 5 to 20 variegated amino acid residue positions, and wherein at least 70% but not all of said variegated amino acid residue positions are located either in the loop, helix surface or linker region of the Alphabody. The invention further provides methods for use of the Alphabody libraries and Alphabodies obtainable by the methods of the invention.

公开(公告)号：US20160326242A1

公开(公告)日：2016-11-10

申请号：US15068178

申请日：2016-03-11

申请人： COMPLIX SA

发明人： Johan Desmet ,  Ignace Lasters ,  Maria Henderikx ,  Anita Wehnert

IPC分类号： C07K16/24 ,  C12N15/10 ,  C07K16/00

CPC分类号： C07K16/244 ,  C07K14/001 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/33 ,  C07K2318/20 ,  C12N15/1037 ,  C12N15/1055 ,  C40B40/10 ,  C40B50/12

摘要： The invention provides single-chain Alphabody library comprising at least 100 different-sequence single-chain Alphabody polypeptides, wherein said Alphabody polypeptides differ from each other in at least one of a defined set of 5 to 20 variegated amino acid residue positions, and wherein at least 70% but not all of said variegated amino acid residue positions are located either in the loop, helix surface or linker region of the Alphabody. The invention further provides methods for use of the Alphabody libraries and Alphabodies obtainable by the methods of the invention.

摘要翻译： 本发明提供了包含至少100个不同序列单链Alphabody多肽的单链Alphabody文库，其中所述Alphabody多肽在至少一个5至20个杂化氨基酸残基位置中的至少一个中彼此不同，并且其中在 至少70％但不是全部所述杂色氨基酸残基位置位于Alphabody的环，螺旋表面或连接区中。 本发明进一步提供了通过本发明方法可获得的使用阿尔法抗体文库和阿尔培布氏体的方法。