公开(公告)号：US20150344926A1

公开(公告)日：2015-12-03

申请号：US14430731

申请日：2013-09-26

申请人： CONTIPRO BIOTECH S.R.O.

发明人： Lenka Knorova ,  Dzianis Smirnou ,  Veronika Leierova ,  Martin Krcmar ,  Lukas Franke ,  Vladimir Velebny

IPC分类号： C12P19/26 ,  C12N9/88

CPC分类号： C12P19/26 ,  C12N9/88 ,  C12P19/04 ,  C12P19/12 ,  C12Y402/02001

摘要： The invention relates to an enzyme which is able to degrade hyaluronic acid and which is produced by fungi of the genus Fistulina (especially Fistulina hepatica). The degradation proceeds by lyase mechanism in which double bonds between C4 and C5 of glucuronic acid are formed. The invention also includes the process of preparation and purification of the enzyme and a possible practical use thereof for the preparation of low-molecular hyaluronan or of cosmetic or pharmaceutical devices. Further, the invention relates to the method of preparation of low-molecular hyaluronan.

摘要翻译： 本发明涉及一种能够降解透明质酸并由瘘管真菌（特别是肝瘘）产生的酶。 通过裂解酶机理进行降解，其中形成葡萄糖醛酸的C4和C5之间的双键。 本发明还包括酶的制备和纯化方法及其可用于制备低分子透明质酸或化妆品或药物装置的实际应用。 此外，本发明涉及低分子透明质酸的制备方法。