公开(公告)号：US20150233804A1

公开(公告)日：2015-08-20

申请号：US14405253

申请日：2013-06-04

申请人： CORNELL UNIVERSITY

发明人： Stephen P. Meisberger ,  Matthew A. Warkentin ,  Jesse B. Hopkins ,  Andrea M. Katz ,  Lois Pollack ,  Robert E. Thorne

IPC分类号： G01N1/42 ,  G01N23/201

CPC分类号： G01N1/42 ,  G01N23/201 ,  G01N2223/3103

摘要： Apparatus and methods for performing small angle X-ray scattering (SAXS) at low (cryogenic) temperatures for determining the structure of and changes in the structure of proteins, DNA, RNA, and other biological molecules and biomolecular assemblies and structures. A cryogenic, small angle X-ray scattering (SAXS) application sample holder, includes a sample cell including a base portion and at least two parallel walls disposed on the base, wherein the sample cell has a liquid volume capacity defined by the walls and the base portion of 0.001 to 10 microliters. A method for performing cryogenic SAXS on a sample includes the steps of providing a sample biomolecule solution containing an aqueous buffer, a biomolecule, and a cryoprotectant agent, wherein the cryoprotectant agent comprises up to 60% (w/w) of the biomolecule solution, and other known components as necessary to solubilize and stabilize the biomolecule, in a sample holder of claim 1 or 18, cryogenically cooling the sample solution in the sample holder at a rate equal to or greater than 100 K/sec without ice formation, and examining the cooled sample using small angle X-ray scattering by passing a beam of X-rays through the sample.

摘要翻译： 用于在低（低温）温度下进行小角度X射线散射（SAXS）的装置和方法，用于确定蛋白质，DNA，RNA和其他生物分子和生物分子组装和结构的结构和结构的变化。 一种低温，小角度X射线散射（SAXS）应用样品架，包括一个样品池，该样品池包括一个基底部分和至少两个设置在基底上的平行壁，其中样品池具有由壁和 基底部分为0.001至10微升。 在样品上进行低温SAXS的方法包括提供含有水性缓冲液，生物分子和冷冻保护剂的样品生物分子溶液的步骤，其中冷冻保护剂包含高达60％（w / w）的生物分子溶液， 和其它已知的成分，以溶解和稳定生物分子，如权利要求1或18所述的样品保持器，以等于或大于100K /秒的速率在样品保持器内低温冷却而不形成冰，并检查 通过使X射线束穿过样品，使用小角度X射线散射的冷却样品。

公开(公告)号：US09927336B2

公开(公告)日：2018-03-27

申请号：US14405253

申请日：2013-06-04

申请人： CORNELL UNIVERSITY

发明人： Stephen P. Meisberger ,  Matthew A. Warkentin ,  Jesse B. Hopkins ,  Andrea M. Katz ,  Lois Pollack ,  Robert E. Thorne

IPC分类号： G01N23/00 ,  G01N1/42 ,  G01N23/201

CPC分类号： G01N1/42 ,  G01N23/201 ,  G01N2223/3103

摘要： Apparatus and methods for performing small angle X-ray scattering (SAXS) at low (cryogenic) temperatures for determining the structure of and changes in the structure of proteins, DNA, RNA, and other biological molecules and biomolecular assemblies and structures. A cryogenic, small angle X-ray scattering (SAXS) application sample holder, includes a sample cell including a base portion and at least two parallel walls disposed on the base, wherein the sample cell has a liquid volume capacity defined by the walls and the base portion of 0.001 to 10 microliters. A method for performing cryogenic SAXS on a sample includes the steps of providing a sample biomolecule solution containing an aqueous buffer, a biomolecule, and a cryoprotectant agent, wherein the cryoprotectant agent comprises up to 60% (w/w) of the biomolecule solution, and other known components as necessary to solubilize and stabilize the biomolecule, in a sample holder of claim 1, cryogenically cooling the sample solution in the sample holder at a rate equal to or greater than 100 K/sec without ice formation, and examining the cooled sample using small angle X-ray scattering by passing a beam of X-rays through the sample.