公开(公告)号：US11573183B2

公开(公告)日：2023-02-07

申请号：US17156027

申请日：2021-01-22

Applicant: CYANAGEN S.r.l.

Inventor： Leopoldo Della Ciana ,  Lorenzo Biagini ,  Thomas Paul Jansen ,  Rossana Perciaccante ,  Manuela Vargiolu ,  Marina Eleonora Vettraino

IPC: G01N21/76 ,  C12Q1/28 ,  G01N21/78 ,  G01N33/573 ,  G01N33/58

Abstract: A kit for performing an assay for determining an analyte in a sample with an extended shelf-life, wherein the kit comprises a chemiluminescent cyclic dihydrazide, an enhancer, a co-enhancer, and a peroxide oxidizer. The kit is useful in blot assays and immunoassays for the detection of proteins and nucleic acid molecules.

公开(公告)号：US11761964B2

公开(公告)日：2023-09-19

申请号：US16749162

申请日：2020-01-22

Applicant: CYANAGEN S.r.l.

Inventor： Leopoldo Della Ciana ,  Lorenzo Biagini ,  Thomas Paul Jansen ,  Rossana Perciaccante ,  Manuela Vargiolu ,  Marina Eleonora Vettraino

IPC: G01N33/58 ,  C09K11/07 ,  G01N33/535

CPC classification number: G01N33/581 ,  C09K11/07 ,  G01N33/582 ,  C09K2211/1037 ,  G01N33/535

Abstract: A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase enzyme or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidizer, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from a 4-dialkylaminopyridine or an N-azole, and

wherein the method comprises the following steps:

i. realizing a chemiluminescent substrate by means of mixing together 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, the enhancer, the co-enhancer and the peroxide oxidizer, and
ii. adding the peroxidase enzyme or a conjugate thereof to the chemiluminescent substrate.

公开(公告)号：US11179481B2

公开(公告)日：2021-11-23

申请号：US16557162

申请日：2019-08-30

Applicant: CYANAGEN S.R.L.

Inventor： Leopoldo Della Ciana ,  Norbert Gretz ,  Rossana Perciaccante ,  Federica Rodeghiero ,  Stefania Geraci ,  Jiaguo Huang ,  Zeneida Herrera Pérez ,  Johannes Pill ,  Stefanie Weinfurter

IPC: A61K49/00 ,  A61M36/14 ,  C09B69/10 ,  C08B37/16 ,  C08L5/16

Abstract: The present invention relates to novel tricarbocyanine-cyclodextrin(s) conjugates useful as markers in the diagnosis of kidney diseases, a diagnostic composition comprising said conjugates, their use and their production.

公开(公告)号：US10441666B2

公开(公告)日：2019-10-15

申请号：US14713459

申请日：2015-05-15

Applicant: CYANAGEN S.R.L.

Inventor： Leopoldo Della Ciana ,  Norbert Gretz ,  Rossana Perciaccante ,  Federica Rodeghiero ,  Stefania Geraci ,  Jiaguo Huang ,  Zeneida Herrera Pérez ,  Johannes Pill ,  Stefanie Weinfurter

IPC: A61K49/00 ,  C09B69/10 ,  C08B37/16 ,  C08L5/16

Abstract: The present invention relates to novel tricarbocyanine-cyclodextrin(s) conjugates useful as markers in the diagnosis of kidney diseases, a diagnostic composition comprising said conjugates, their use and their production.

公开(公告)号：US10160861B2

公开(公告)日：2018-12-25

申请号：US15458801

申请日：2017-03-14

Applicant: CYANAGEN S.r.l.

Inventor： Thomas Paul Jansen ,  Giacomo Rodeghiero ,  Dario Foglietta ,  Rossana Perciaccante ,  Leopoldo Della Ciana

IPC: C07D311/08 ,  C07D409/04 ,  C09B57/02 ,  C07K16/28 ,  G01N33/52 ,  C07D409/14 ,  A61K47/54

Abstract: The present invention describes novel 7-alkylamino-3-(thienyl) coumarin fluorescent dyes of formula (I) These dyes are water soluble, can be excited by the 405 nm excitation source and exhibit a large Stokes shift (≥80 nm). Furthermore, the dyes possess a reactive group for the labeling of biomolecules or other analytes.