公开(公告)号：US20220305529A1

公开(公告)日：2022-09-29

申请号：US17706432

申请日：2022-03-28

申请人： CYTONOME/ST, LLC

发明人： Gary Durack ,  William J. Williams, V

IPC分类号： B07C5/342

摘要： Systems and methods for particle sorting are presented including a monitoring system downstream of a particle separator or sorter. The system can utilize the monitoring system to adjust or calibrate operational parameters of the system in real time. When a particle of interest is mis-sorted, the probability is high that the particle of interest has been sorted into a non-targeted sortable unit that was adjacent in sequence to the sortable unit that was expected to include the particle of interest. The monitoring system monitors non-targeted sortable units in the system that were adjacent in sequence to targeted sortable units that are predicted to contain particles of interest. Signals from the monitoring system enable automated adjustment or calibration of operational parameters of the system such as sort delay or purity mask parameters.

公开(公告)号：US11858008B2

公开(公告)日：2024-01-02

申请号：US17706432

申请日：2022-03-28

申请人： CYTONOME/ST, LLC

发明人： Gary Durack ,  William J. Williams, V

IPC分类号： B07C5/34 ,  B07C5/342 ,  B07C5/36

CPC分类号： B07C5/3425 ,  B07C5/362

摘要： Systems and methods for particle sorting are presented including a monitoring system downstream of a particle separator or sorter. The system can utilize the monitoring system to adjust or calibrate operational parameters of the system in real time. When a particle of interest is mis-sorted, the probability is high that the particle of interest has been sorted into a non-targeted sortable unit that was adjacent in sequence to the sortable unit that was expected to include the particle of interest. The monitoring system monitors non-targeted sortable units in the system that were adjacent in sequence to targeted sortable units that are predicted to contain particles of interest. Signals from the monitoring system enable automated adjustment or calibration of operational parameters of the system such as sort delay or purity mask parameters.

公开(公告)号：US20240142368A1

公开(公告)日：2024-05-02

申请号：US18384641

申请日：2023-10-27

申请人： CYTONOME/ST, LLC

发明人： Gary Durack ,  William J. Williams, V

IPC分类号： G01N15/14

CPC分类号： G01N15/1434 ,  G01N15/1429 ,  G01N2015/1452

摘要： A method, system and apparatus for selecting cells is disclosed. An example embodiment provides spatial information denoting the location, on a four part array of sensors, referred to as a quad array, of the center, of each particle that passes through the focused laser beam in a cell sorter. The center of a particle is commonly determined by the average fluorescence intensity within the field of view of a Side Fluorescence (SFL) collection lens. Fluorescence collected by an SFL lens from a particle having any orientation and any position in the core stream produces a spot large enough to illuminate all four segments of a quad array. By independently measuring the fluorescence intensity recorded by each of the four array segments for a particle, it is possible to calculate the x-y coordinate position across the quad array segments of the average total fluorescence emission collected from that particle.

公开(公告)号：US20240042491A1

公开(公告)日：2024-02-08

申请号：US18382268

申请日：2023-10-20

申请人： CYTONOME/ST, LLC

发明人： Gary Durack ,  William J. Williams, V

IPC分类号： B07C5/342

CPC分类号： B07C5/3425 ,  B07C5/362

摘要： Systems and methods for particle sorting are presented including a monitoring system downstream of a particle separator or sorter. The system can utilize the monitoring system to adjust or calibrate operational parameters of the system in real time. When a particle of interest is mis-sorted, the probability is high that the particle of interest has been sorted into a non-targeted sortable unit that was adjacent in sequence to the sortable unit that was expected to include the particle of interest. The monitoring system monitors non-targeted sortable units in the system that were adjacent in sequence to targeted sortable units that are predicted to contain particles of interest. Signals from the monitoring system enable automated adjustment or calibration of operational parameters of the system such as sort delay or purity mask parameters.