公开(公告)号：US20230408504A1

公开(公告)日：2023-12-21

申请号：US18167362

申请日：2023-02-10

Applicant: California Institute of Technology

Inventor： Mitchell Guttman ,  Andrew A. Perez ,  Mario R. Blanco ,  Isabel N. Goronzy ,  James K. Guo

IPC: G01N33/543

CPC classification number: G01N33/54353 ,  G01N33/54326 ,  G01N33/54346

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in generating barcoded detection particles. Each barcoded detection particle can comprise a particle associated with an antigen-binding protein and a plurality of barcoding oligonucleotides. The plurality of barcoding oligonucleotides can comprise a first ligand. The particle can comprise a second ligand. The plurality of barcoding oligonucleotides can be associated with the particle via a multivalent binding agent comprising two or more binding moieties capable of binding the first ligand and/or the second ligand. There are provided, in some embodiments, methods for detecting interactions between nucleic acid molecules and proteins of interest. Methods for detecting interactions between ribonucleic acid molecules and RNA-binding proteins (RBPs) are also provided herein.