公开(公告)号：US20170292958A1

公开(公告)日：2017-10-12

申请号：US15495414

申请日：2017-04-24

Applicant: California Institute of Technology

Inventor： Daniela C. Dieterich ,  David Tirrell ,  Erin Schuman ,  Aaron J. Link

IPC: G01N33/68

CPC classification number: G01N33/6842 ,  C07K7/06 ,  G01N33/5091 ,  G01N33/582 ,  G01N33/6848

Abstract: The invention provides methods, reagents and systems for incorporating non-natural amino acids into proteins, preferably in vivo, using the endogenous protein synthesis machinery of an organism. The incorporated non-natural amino acids contain reactive groups for further chemical reagents, which may serve as a “handle” to enrich the proteins or fragments thereof in a number of uses, such as proteomic analysis, imaging of diseased tissues/cells, etc.

公开(公告)号：US20210223252A1

公开(公告)日：2021-07-22

申请号：US17067028

申请日：2020-10-09

Applicant: California Institute of Technology

Inventor： Daniela C. Dieterich ,  David Tirrell ,  Erin Schuman ,  Aaron J. Link

IPC: G01N33/58 ,  G01N33/68 ,  G01N33/50 ,  C07K7/06

Abstract: The invention provides methods, reagents and systems for incorporating non-natural amino acids into proteins, preferably in vivo, using the endogenous protein synthesis machinery of an organism. The incorporated non-natural amino acids contain reactive groups for further chemical reagents, which may serve as a “handle” to enrich the proteins or fragments thereof in a number of uses, such as proteomic analysis, imaging of diseased tissues/cells, etc.