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公开(公告)号：US09133457B2
公开(公告)日：2015-09-15
申请号：US14455581
申请日：2014-08-08
Applicant: California Institute of Technology
Inventor： Inchan Kwon , David Tirrell
CPC classification number: C12N15/113 , C07H21/02 , C12N9/93 , C12N15/70 , C12N2310/10 , C12P21/00 , C12P21/02
Abstract: The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs.
Abstract translation: 本发明提供了一种通过利用修饰的氨酰-tRNA合成酶将非标准氨基酸掺入蛋白质的方法，以将非标准氨基酸装入修饰的tRNA，其与通常形成摆动碱基的密码子形成严格的沃森 - 克里克碱基配对， 与天然tRNA配对。
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公开(公告)号：US20130210073A1
公开(公告)日：2013-08-15
申请号：US13730116
申请日：2012-12-28
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Inchan Kwon , David Tirrell
CPC classification number: C12N15/113 , C07H21/02 , C12N9/93 , C12N15/70 , C12N2310/10 , C12P21/00 , C12P21/02
Abstract: The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs.
Abstract translation: 本发明提供了一种通过利用修饰的氨酰-tRNA合成酶将非标准氨基酸掺入蛋白质的方法，以将非标准氨基酸装入修饰的tRNA，其与通常形成摆动碱基的密码子形成严格的沃森 - 克里克碱基配对， 与天然tRNA配对。
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公开(公告)号：US20170292958A1
公开(公告)日：2017-10-12
申请号：US15495414
申请日：2017-04-24
Applicant: California Institute of Technology
Inventor： Daniela C. Dieterich , David Tirrell , Erin Schuman , Aaron J. Link
IPC: G01N33/68
CPC classification number: G01N33/6842 , C07K7/06 , G01N33/5091 , G01N33/582 , G01N33/6848
Abstract: The invention provides methods, reagents and systems for incorporating non-natural amino acids into proteins, preferably in vivo, using the endogenous protein synthesis machinery of an organism. The incorporated non-natural amino acids contain reactive groups for further chemical reagents, which may serve as a “handle” to enrich the proteins or fragments thereof in a number of uses, such as proteomic analysis, imaging of diseased tissues/cells, etc.
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Patent Agency Ranking
公开(公告)号：US08980581B2
公开(公告)日：2015-03-17
申请号：US13969368
申请日：2013-08-16
Applicant: California Institute of Technology
Inventor： Pin Wang , Inchan Kwon , Soojin Son , Yi Tang , David Tirrell
Abstract: The invention provides certain embodiments relating to methods and compositions for incorporating non-natural amino acids into a polypeptide or protein by utilizing a mutant or modified aminoacyl-tRNA synthetase to charge the non-natural amino acid to a the corresponding tRNA. In certain embodiments, the tRNA is also modified such that the complex forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with unmodified tRNA/aminoacyl-tRNA synthetase pairs.
Abstract translation: 本发明提供了涉及将非天然氨基酸并入多肽或蛋白质的方法和组合物的某些实施方案，所述方法和组合物通过利用突变或修饰的氨酰-tRNA合成酶将非天然氨基酸加载至相应的tRNA。 在某些实施方案中，tRNA也被修饰，使得复合物与通常与未修饰的tRNA /氨酰-tRNA合成酶对形成摆动碱基配对的密码子形成严格的沃森 - 克里克碱基配对。
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公开(公告)号：US20140045261A1
公开(公告)日：2014-02-13
申请号：US13969368
申请日：2013-08-16
Applicant: California Institute of Technology
Inventor： Pin Wang , Inchan Kwon , Soojin Son , Yi Tang , David Tirrell
Abstract: The invention provides certain embodiments relating to methods and compositions for incorporating non-natural amino acids into a polypeptide or protein by utilizing a mutant or modified aminoacyl-tRNA synthetase to charge the non-natural amino acid to a the corresponding tRNA. In certain embodiments, the tRNA is also modified such that the complex forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with unmodified tRNA/aminoacyl-tRNA synthetase pairs.
Abstract translation: 本发明提供了涉及将非天然氨基酸并入多肽或蛋白质的方法和组合物的某些实施方案，所述方法和组合物通过利用突变或修饰的氨酰-tRNA合成酶将非天然氨基酸加载至相应的tRNA。 在某些实施方案中，tRNA也被修饰，使得复合物与通常与未修饰的tRNA /氨酰-tRNA合成酶对形成摆动碱基配对的密码子形成严格的沃森 - 克里克碱基配对。
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公开(公告)号：US20210223252A1
公开(公告)日：2021-07-22
申请号：US17067028
申请日：2020-10-09
Applicant: California Institute of Technology
Inventor： Daniela C. Dieterich , David Tirrell , Erin Schuman , Aaron J. Link
Abstract: The invention provides methods, reagents and systems for incorporating non-natural amino acids into proteins, preferably in vivo, using the endogenous protein synthesis machinery of an organism. The incorporated non-natural amino acids contain reactive groups for further chemical reagents, which may serve as a “handle” to enrich the proteins or fragments thereof in a number of uses, such as proteomic analysis, imaging of diseased tissues/cells, etc.
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公开(公告)号：US20140342451A1
公开(公告)日：2014-11-20
申请号：US14455581
申请日：2014-08-08
Applicant: California Institute of Technology
Inventor： Inchan Kwon , David Tirrell
IPC: C12N15/113
CPC classification number: C12N15/113 , C07H21/02 , C12N9/93 , C12N15/70 , C12N2310/10 , C12P21/00 , C12P21/02
Abstract: The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs.
Abstract translation: 本发明提供了一种通过利用修饰的氨酰-tRNA合成酶将非标准氨基酸掺入蛋白质的方法，以将非标准氨基酸装入修饰的tRNA，其与通常形成摆动碱基的密码子形成严格的沃森 - 克里克碱基配对， 与天然tRNA配对。
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公开(公告)号：US08835162B2
公开(公告)日：2014-09-16
申请号：US13730116
申请日：2012-12-28
Applicant: California Institute of Technology
Inventor： Inchan Kwon , David Tirrell
IPC: C12N15/70
CPC classification number: C12N15/113 , C07H21/02 , C12N9/93 , C12N15/70 , C12N2310/10 , C12P21/00 , C12P21/02
Abstract: The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs.
Abstract translation: 本发明提供了一种通过利用修饰的氨酰-tRNA合成酶将非标准氨基酸掺入蛋白质的方法，以将非标准氨基酸装入修饰的tRNA，其与通常形成摆动碱基的密码子形成严格的沃森 - 克里克碱基配对， 与天然tRNA配对。
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