公开(公告)号：US20140255991A1

公开(公告)日：2014-09-11

申请号：US14151686

申请日：2014-01-09

Applicant: California Institute of Technology

Inventor： David A. Tirrell ,  Kristi Lynn Kiick

IPC: C12N9/00 ,  C12P21/00

CPC classification number: C12P21/00 ,  C12N9/003 ,  C12N9/93 ,  C12N15/67 ,  C12P21/02

Abstract: Methods for producing modified polypeptides containing amino acid analogues are disclosed. The invention further provides purified dihydrofolate reductase polypeptides, produced by the methods of the invention, in which the methionine residues have been replaced with homoallylglycine, homoproparglycine, norvaline, norleucine, cis-crotylglycine, trans-crotylglycine, 2-aminoheptanoic acid, 2-butynylglycine and allylglycine.

Abstract translation: 公开了生产含有氨基酸类似物的修饰多肽的方法。 本发明进一步提供了通过本发明方法制备的纯化的二氢叶酸还原酶多肽，其中甲硫氨酸残基已经被均一甘氨酸，高丙氨酸，正缬氨酸，正亮氨酸，顺式 - 巴豆基甘氨酸，反 - 巴豆基甘氨酸，2-氨基庚酸，2-丁炔基甘氨酸 和烯丙基甘氨酸。