公开(公告)号：US20220378828A1

公开(公告)日：2022-12-01

申请号：US17621182

申请日：2020-06-29

Applicant: CANCER RESEARCH TECHNOLOGY LIMITED

Inventor： Francis Jay Mussai ,  Carmela De Santo

IPC: A61K35/17 ,  C07K14/725 ,  C07K14/705 ,  C07K16/30 ,  C07K16/28 ,  C07K16/18 ,  C12N9/78

Abstract: The invention relates to fusion target-binding proteins, such as chimeric antigen receptors (CARs), that comprise a target binding moiety, an intracellular signalling region, and an arginase domain. These proteins confer advantages that include improved cell killing and increased proliferation. The invention also relates to nucleic acids encoding the fusion target-binding proteins and cells expressing such proteins. The invention relates to pharmaceutical compositions, medical uses, and methods of treatment, all using the fusion target-binding proteins, cells, or nucleic acids disclosed. The medical uses and methods of treatment are of particular benefit in cancer therapy.

公开(公告)号：US20240000835A1

公开(公告)日：2024-01-04

申请号：US18038581

申请日：2021-11-24

Applicant: Cancer Research Technology Limited

Inventor： Francis MUSSAI ,  Carmela De Santo

IPC: A61K35/17 ,  C12N5/0783 ,  C07K14/435 ,  C07K16/30 ,  C07K16/28 ,  A61P35/00 ,  A61K39/00

CPC classification number: A61K35/17 ,  C12N5/0636 ,  C12N5/0646 ,  C07K14/435 ,  C07K16/3084 ,  C07K16/2803 ,  A61P35/00 ,  A61K39/4631 ,  A61K39/4613 ,  A61K39/4611 ,  A61K39/464411 ,  C07K16/2863 ,  A61K39/464404 ,  A61K39/464471 ,  A61K2239/28 ,  A61K2239/13

Abstract: The invention relates to nucleic acid constructs comprising a combination of a first sequence encoding a CAR and second sequence encoding an amino acid transporter, and to cells comprising such nucleic acid constructs. It also relates to methods of manufacturing said cells, and to pharmaceutical compositions comprising said nucleic acid constructs or cells, for use in the treatment of diseases with cellular amino-acid depletion such as cancer.

公开(公告)号：US20200308557A1

公开(公告)日：2020-10-01

申请号：US16955684

申请日：2018-12-24

Applicant: CANCER RESEARCH TECHNOLOGY LIMITED

Inventor： Francis Jay Mussai ,  Carmela De Santo ,  Steven Lee

IPC: C12N9/10 ,  A61P35/00 ,  A61P31/12 ,  C12N9/00 ,  A61K38/53 ,  A61K38/45

Abstract: Provided are fusion target-binding proteins comprising a target binding moiety, an intracellular signalling region and a domain that promotes synthesis of arginine or an arginine precursor. The domain may be an enzyme domain such as an argininosuccinate synthase (ASS-1) enzyme domain, or an ornithine transcarbamylase (OTC) enzyme domain. Also provided are cells comprising such a fusion target-binding protein (for example cells that express the fusion target-binding protein), and nucleic acids encoding such fusion target-binding proteins. The invention also provides fusion target-binding proteins comprising a target binding moiety, an intracellular signalling region and a domain that promotes synthesis of tryptophan or a tryptophan precursor. Pharmaceutical compositions, medical uses, and methods of treatment, all using the fusion target-binding proteins, cells, or nucleic acids are disclosed. The proteins, cells, nucleic acids and pharmaceutical compositions may be used in the prevention and/or treatment of cancer, such as neuroblastoma or acute myeloid leukaemia.