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公开(公告)号：US20170123198A1
公开(公告)日：2017-05-04
申请号：US15337156
申请日：2016-10-28
发明人： Wolfgang Singer , Aksel Goehnermeier , Guenter Rudolph , Johannes Ruoff , Christian Dietrich , Rong Dong
CPC分类号： G02B21/28 , G01N21/0332 , G01N2021/0335 , G02B21/02 , G02B21/18 , G02B21/26 , G02B21/33 , G02B21/362
摘要： The invention relates to a device for optical examination of a specimen with a cryo-immersion objective, having a stative, to which the cryo-immersion objective is fixed, wherein the cryo-immersion objective has a plurality of optical components, in particular lenses, and wherein the cryo-immersion objective has an optical front component which is in contact during operation with a coolable immersion liquid, having a specimen carrier for a specimen to be examined, having means for providing a cooled immersion liquid between the optical front component and the specimen to be examined on or against the specimen carrier. The device is characterised in that insulating means are present for interrupting a heat transition between the stative and the optical front component. The invention also relates to a method for examining a specimen, wherein with a device according to the invention a plurality of microscopic images are recorded, wherein for each of the individual images a different offset between a main housing and a separate housing is set. Finally the invention relates to a method for transferring a device according to the invention into an operation-ready state, wherein the components, cooled in operation, of the cryo-immersion objective, in particular the optical front component, are cooled with a coolant, in particular with liquid nitrogen, wherein the immersion liquid is cooled with a coolant, in particular with liquid nitrogen, and wherein thereafter the cooled components of the cryo-immersion objective are brought into contact with the immersion liquid.
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公开(公告)号：US10345570B2
公开(公告)日：2019-07-09
申请号：US15337156
申请日：2016-10-28
发明人： Wolfgang Singer , Aksel Goehnermeier , Guenter Rudolph , Johannes Ruoff , Christian Dietrich , Rong Dong
IPC分类号： G01N21/03 , G02B21/02 , G02B21/18 , G01B21/26 , G02B21/28 , G02B21/33 , G02B21/36 , G02B21/26
摘要： The invention relates to a device for optical examination of a specimen with a cryo-immersion objective, having a stative, to which the cryo-immersion objective is fixed, wherein the cryo-immersion objective has a plurality of optical components, in particular lenses, and wherein the cryo-immersion objective has an optical front component which is in contact during operation with a coolable immersion liquid, having a specimen carrier for a specimen to be examined, having means for providing a cooled immersion liquid between the optical front component and the specimen to be examined on or against the specimen carrier. The device is characterized in that insulating means are present for interrupting a heat transition between the stative and the optical front component. The invention also relates to a method for examining a specimen, wherein with a device according to the invention a plurality of microscopic images are recorded, wherein for each of the individual images a different offset between a main housing and a separate housing is set. Finally the invention relates to a method for transferring a device according to the invention into an operation-ready state, wherein the components, cooled in operation, of the cryo-immersion objective, in particular the optical front component, are cooled with a coolant, in particular with liquid nitrogen, wherein the immersion liquid is cooled with a coolant, in particular with liquid nitrogen, and wherein thereafter the cooled components of the cryo-immersion objective are brought into contact with the immersion liquid.
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