公开(公告)号：US10754136B2

公开(公告)日：2020-08-25

申请号：US15127101

申请日：2015-03-19

申请人： Carl Zeiss Microscopy GmbH

发明人： Nils Langholz ,  Viktor Drescher ,  Helmut Lippert ,  Wolfgang Schwerdtfeger

IPC分类号： G02B21/00 ,  G02B21/36 ,  G01B11/24 ,  G02B21/06

摘要： A confocal microscope and an associated method for determining a topography of a sample by implementing a correlative spinning disk microscopy is provided. The method includes placing a sample on an object stage of the microscope. Either the object stage is moved vertically to determine the topography of the sample, while first and second images of the sample are captured in an alternating manner. A vertical focus position is stored as metadata for each image. Two first or second images are interpolated to give an intermediate image. A confocal image for a defined vertical position is generated by calculating the intermediate image with the second or first image at the position.