公开(公告)号：US20240182961A1

公开(公告)日：2024-06-06

申请号：US18536784

申请日：2023-12-12

申请人： Carlsberg A/S

发明人： Toni Wendt ,  Ole Olsen ,  Søren Knudsen ,  Hanne Cecille Thomsen ,  Birgitte Skadhauge ,  Magnus Wohlfahrt Rasmussen ,  Massimiliano Carciofi ,  Alexander Striebeck

IPC分类号： C12Q1/686 ,  A01H6/46 ,  C12N15/01 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6876 ,  C40B30/00 ,  C40B40/02

CPC分类号： C12Q1/686 ,  A01H6/4624 ,  C12N15/01 ,  C12N15/1024 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6876 ,  C40B30/00 ,  C40B40/02

摘要： In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR—based systems and a set-up to propagate and analyze identified, mutated organisms.

公开(公告)号：US11884972B2

公开(公告)日：2024-01-30

申请号：US16311450

申请日：2017-06-23

申请人： Carlsberg A/S

发明人： Toni Wendt ,  Ole Olsen ,  Søren Knudsen ,  Hanne Cecille Thomsen ,  Birgitte Skadhauge ,  Magnus Wohlfahrt Rasmussen ,  Massimiliano Carciofi ,  Alexander Striebeck

IPC分类号： C12Q1/68 ,  C12Q1/686 ,  C12N15/01 ,  C12N15/10 ,  A01H6/46 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6876 ,  C40B30/00 ,  C40B40/02

CPC分类号： C12Q1/686 ,  A01H6/4624 ,  C12N15/01 ,  C12N15/1024 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6876 ,  C40B30/00 ,  C40B40/02

摘要： In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.

公开(公告)号：US20190194723A1

公开(公告)日：2019-06-27

申请号：US16311450

申请日：2017-06-23

申请人： Carlsberg A/S

发明人： Toni Wendt ,  Ole Olsen ,  Søren Knudsen ,  Hanne Cecille Thomsen ,  Birgitte Skadhauge ,  Magnus Wohlfahrt Rasmussen ,  Massimiliano Carciofi ,  Alexander Striebeck

IPC分类号： C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6827 ,  A01H6/46 ,  A01H5/10

CPC分类号： C12Q1/686 ,  A01H5/10 ,  A01H6/4624 ,  C12N15/01 ,  C12N15/1024 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6876 ,  C40B30/00 ,  C40B40/02

摘要： In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.