公开(公告)号：US20170158836A1

公开(公告)日：2017-06-08

申请号：US15039219

申请日：2014-11-25

Applicant: Carnegie Mellon University

Inventor： Krzysztof Matyjaszewski ,  Saadyah Averick ,  Hongkun He

IPC: C08J9/26 ,  C02F3/34 ,  C12N9/96 ,  C08J9/00 ,  C08J9/36

CPC classification number: C08J9/26 ,  B01D71/40 ,  B01D71/44 ,  B01D71/52 ,  B01D71/82 ,  B01D2323/30 ,  C02F3/342 ,  C08J9/0061 ,  C08J9/36 ,  C08J2201/0462 ,  C08J2205/022 ,  C08J2205/05 ,  C08J2333/06 ,  C08J2333/12 ,  C08J2335/02 ,  C08J2489/00 ,  C12N9/96 ,  C12Y304/21004

Abstract: A three-dimensionally ordered macroporous hydrogel for immobilizing a selected bioresponsive molecule and method of making are disclosed. The three-dimensionally ordered macroporous hydrogel comprises a crosslinked polymer that has a system of interconnected pores. The interconnected pores have a uniform pore size in the range of 50 to 5000 nm, and a plurality of first pore functional groups. The plurality of first pore functional groups is selected to immobilize a selected bioresponsive molecule. Examples of bioresponsive molecules include an enzyme; a molecule for: a protein scaffold, solid phase synthesis, nucleic acid synthesis, polypeptide synthesis, analyte detection, adsorption of analytes and measuring analyte concentrations, organic synthesis, and degradation of biologically active agents in wastewater. A method includes forming a colloidal crystal template, polymerizing a hydrogel within the pores of the colloidal crystal template, and selectively removing the colloidal crystal template. The hydrogel can be polymerized using CRP, ATRP and FRP polymerization processes.