公开(公告)号：US09683011B2

公开(公告)日：2017-06-20

申请号：US14720331

申请日：2015-05-22

Applicant: Case Western Reserve University

Inventor： Gary E. Wnek ,  Linghui Meng

IPC: C07K1/107 ,  C07K1/36 ,  A61L27/22 ,  C07K14/78 ,  D01F1/10 ,  D01F4/00

CPC classification number: C07K1/1075 ,  A61L27/22 ,  C07K1/107 ,  C07K1/36 ,  C07K14/78 ,  D01F1/10 ,  D01F4/00

Abstract: A method of forming a cross-linked protein structures includes preparing a solution of protein dissolved in a benign solvent and forming an intermediate protein structure from the solution. The intermediate protein structure can be cross-linked by providing for a specific ratio of chemical cross-linking agents to form the cross-linked protein structure. The solution can be prepared by adding a cross-linker of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) at a ratio of two-to-one of NHS to EDC to alcohol. PBS buffer (20×) can be added to the solution until the volume ratio of PBS buffer (20×) to alcohol is about one-to-one. About 16 percent by weight of protein can be dissolved in the solution. The solution can be electrospun to form an intermediate protein structure. After a period of time, the protein structure can be cross-linked to form the cross-linked protein structure.