公开(公告)号：US07553658B2

公开(公告)日：2009-06-30

申请号：US10526089

申请日：2003-06-17

申请人： Cecilia Jansson Kepka ,  Jenny Rhodin Edsō ,  Folke Tjerneld

发明人： Cecilia Jansson Kepka ,  Jenny Rhodin Edsō ,  Folke Tjerneld

IPC分类号： C12N1/08 ,  C12N15/64 ,  C12N15/10

CPC分类号： C12N15/1006

摘要： The present invention is a method for the purification of plasmid DNA comprising to provide a composition comprising a first polymer having inverse solubility characteristics and a second polymer immiscible in the first polymer; contacting said solution with an aqueous solution comprising plasmid DNA; providing phase separation and isolating the aqueous phase; and increasing the temperature of the isolated phase to a temperature above the cloud point of the first polymer and below the temperature where plasmid DNA is degraded and subsequently isolating the aqueous phase so formed. The invention also encompasses a kit for purification of plasmid DNA as described above.

摘要翻译： 本发明是用于纯化质粒DNA的方法，其包括提供包含具有相反溶解度特征的第一聚合物和在第一聚合物中不混溶的第二聚合物的组合物; 使所述溶液与包含质粒DNA的水溶液接触; 提供相分离和分离水相; 并将分离相的温度升高到高于第一聚合物的浊点的温度，并且低于质粒DNA降解的温度，随后分离如此形成的水相。 本发明还包括如上所述的用于纯化质粒DNA的试剂盒。