公开(公告)号：US11969702B2

公开(公告)日：2024-04-30

申请号：US15927057

申请日：2018-03-20

Applicant: Celldom, Inc. ,  The Regents of the University of California

Inventor： Natalia Tkachenko ,  Eugene Tkachenko ,  Alexander Groisman ,  Edgar Gutierrez

IPC: B01J19/00 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/02 ,  C40B40/06 ,  G01N33/50 ,  G01N33/543

CPC classification number: B01J19/0046 ,  B01L3/5085 ,  C12N15/1013 ,  C12Q1/02 ,  C40B40/06 ,  G01N33/5005 ,  G01N33/54366 ,  B01J2219/00317 ,  B01J2219/005 ,  B01J2219/00648 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0858

Abstract: The present invention provides methods, systems and kits for performing microwell analysis in sealed small volumes. The microwells of the invention are sealed through the pairing of microwell and bead geometry wherein the diameter of the beads is greater than the diameter of the bottom of the microwells. Loading the beads into the microwells seals samples within an analytical space between the bottom of a nested bead and the bottom of the subject microwell.

公开(公告)号：US20240060961A1

公开(公告)日：2024-02-22

申请号：US18497955

申请日：2023-10-30

Applicant: The Regents of the University of California ,  CELLDOM, Inc.

Inventor： Eugene Tkachenko ,  Natalia Tkachenko ,  Edgar Gutierrez ,  Alexander Groisman

IPC: G01N33/50 ,  G01N33/543 ,  G01N33/58 ,  B01L3/00

CPC classification number: G01N33/5008 ,  G01N33/54306 ,  G01N33/585 ,  B01L3/5085 ,  G01N2500/02 ,  G01N2500/10 ,  B01L2300/021 ,  B01L3/50853 ,  B01L2200/0668 ,  B01L2300/123 ,  B01L2200/0689 ,  B01L2300/0893

Abstract: Provided are methods and compositions for cell-based drug screening with high-density arrays of conical microwells and slow-drug-release beads.

公开(公告)号：US20240342678A1

公开(公告)日：2024-10-17

申请号：US18620775

申请日：2024-03-28

Applicant: Celldom, Inc. ,  The Regents of the University of California

Inventor： Natalia Tkachenko ,  Eugene Tkachenko ,  Alexander Groisman ,  Edgar Gutierrez

IPC: B01J19/00 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/02 ,  C40B40/06 ,  G01N33/50 ,  G01N33/543

CPC classification number: B01J19/0046 ,  B01L3/5085 ,  C12N15/1013 ,  C12Q1/02 ,  C40B40/06 ,  G01N33/5005 ,  G01N33/54366 ,  B01J2219/00317 ,  B01J2219/005 ,  B01J2219/00648 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0858

Abstract: The present invention provides methods, systems and kits for performing microwell analysis in sealed small volumes. The microwells of the invention are sealed through the pairing of microwell and bead geometry wherein the diameter of the beads is greater than the diameter of the bottom of the microwells. Loading the beads into the microwells seals samples within an analytical space between the bottom of a nested bead and the bottom of the subject microwell.

公开(公告)号：US20190137481A1

公开(公告)日：2019-05-09

申请号：US16178448

申请日：2018-11-01

Applicant: The Regents of the University of California ,  MuWells, Inc.

Inventor： Eugene Tkachenko ,  Natalia Tkachenko ,  Edgar Gutierrez ,  Alexander Groisman

IPC: G01N33/50 ,  G01N33/543

Abstract: Provided are methods and compositions for cell-based drug screening with high-density arrays of conical microwells and slow-drug-release beads.

公开(公告)号：US09039171B2

公开(公告)日：2015-05-26

申请号：US13869762

申请日：2013-04-24

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Alexander Groisman

IPC: G02C7/04

CPC classification number: G02C7/04 ,  G02C7/047 ,  G02C7/049 ,  G02C2202/16

Abstract: A contact lens assembly includes a proximal layer formed from a gas-permeable material, a distal layer with an optical element, an air cavity disposed between the proximal layer and the distal layer, wherein oxygen concentration across the air cavity is substantially uniform, and an gas transport region surrounding the air cavity for communicating gas between the air cavity and atmosphere. The contact lens assembly may further include a plurality of fins disposed within the air cavity where the plurality of fins has openings therethrough. One or both of the upper and lower surfaces of the air cavity may have an anti-reflection coating formed thereon.

Abstract translation: 隐形眼镜组件包括由气体可渗透材料形成的近侧层，具有光学元件的远侧层，设置在近端层和远侧层之间的空气腔，其中空气腔上的氧气浓度基本上均匀， 围绕空气腔的气体输送区域，用于在空气腔和大气之间连通气体。 隐形眼镜组件还可以包括设置在空气腔内的多个翅片，其中多个翅片具有穿过其中的开口。 空气腔的上表面和下表面中的一个或两个可以在其上形成防反射涂层。

公开(公告)号：US20240344007A1

公开(公告)日：2024-10-17

申请号：US18294270

申请日：2022-08-05

Applicant: The Regents of the University of California

Inventor： Alexander Groisman

IPC: C12M1/12 ,  C12M1/32

CPC classification number: C12M25/02 ,  C12M23/12

Abstract: Compositions and methods are described herein that are useful for microbial culture of “uncultivable” microorganisms. The disclosure specifically ad-dresses the issues pertaining to efficient extraction of the cultivated microorganisms from growth chambers. The compositions and methods described herein are particularly useful for liquid medium but are also useful for solid media such as hydrogels or any such comparable media. In addition, the disclosure relates to, among other things, preventing cross-contamination and contamination from environmental microorganisms, especially during incubation in various natural and man-made environments and during extraction from growth chambers. Since these uncultivatable microorganisms are not initially cultured in synthetic laboratory medium, more care may be necessary to maintain sterility, and prevent the cross-contamination with environmental microorganisms.