公开(公告)号：US20130209437A1

公开(公告)日：2013-08-15

申请号：US13744068

申请日：2013-01-17

Applicant: Cellectis

Inventor： Frederic Paques

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/90 ,  A01N63/00

CPC classification number: C12N9/22 ,  A01N63/00 ,  C12N15/1086 ,  C12N15/902

Abstract: A method for engineering I-CreI homing endonuclease variants able to cleave mutant I-CreI sites having variation in positions ±8 to ±10. A I-CreI homing endonuclease variant obtainable by said method, a vector encoding said variant, a cell, an animal or a plant modified by said vector. Use of said I-CreI endonuclease variant and derived products for genetic engineering, genome therapy and antiviral therapy.

Abstract translation: 用于工程化I-CreI归巢内切核酸酶变体的方法，其能够切割具有±8至±10位置变异的突变I-CreI位点。 通过所述方法可获得的I-CreI归巢内切核酸酶变体，编码所述变体的载体，细胞，动物或由所述载体修饰的植物。 使用所述I-CreI内切核酸酶变体和衍生产物用于基因工程，基因组治疗和抗病毒治疗。