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    Method and Assay for Glycosylation Pattern Detection Related to Cell State
    5.
    发明申请
    Method and Assay for Glycosylation Pattern Detection Related to Cell State 审中-公开
    与细胞状态相关的糖基化模式检测的方法和测定

    公开(公告)号：US20120165206A1

    公开(公告)日：2012-06-28

    申请号：US12309219

    申请日：2007-07-11

    申请人： Rakefet Rosenfeld ,  Noa Zalle ,  Dorit Landstein ,  Albena Samokovlisky ,  Ronny Aloni ,  Ruth Maya ,  Yeshayahu Yakir

    发明人： Rakefet Rosenfeld ,  Noa Zalle ,  Dorit Landstein ,  Albena Samokovlisky ,  Ronny Aloni ,  Ruth Maya ,  Yeshayahu Yakir

    IPC分类号： C40B30/04 ,  G01N33/53

    CPC分类号： G01N33/5091 ,  G01N2333/42 ,  G01N2333/4724 ,  G01N2800/52

    摘要： A method and assay for characterizing populations of cells according to their glycosylation pattern, particularly for distinguishing between cell populations. In preferred embodiments the present invention is able to determine the state of a stem cell (ie differentiated or undifferentiated) and/or the state of a cancer cell, for example with regard to malignancy. Preferably the present invention is also able to determine whether a patient is likely to respond to a drug according to the glycosylation pattern of a sample of cancer cells taken from the patient (or alternatively examined while in the patient, as described in greater detail below). Also optionally, it may be used to analyze a cell population before and after treatment with a drug for example.

    摘要翻译： 根据其糖基化模式来表征细胞群体的方法和测定法，特别是用于区分细胞群体。 在优选的实施方案中，本发明能够确定例如关于恶性肿瘤的干细胞（即分化或未分化）和/或癌细胞的状态的状态。 优选地，本发明还能够确定患者是否可能根据从患者取得的癌细胞的样品的糖基化模式（或在患者中进行检查，如下文更详细描述）中对药物作出反应） 。 也可选地，其可以用于分析用例如药物治疗之前和之后的细胞群体。

    Identifying pharmacophore containing combinations of scaffold molecules and substituents from a virtual library
    7.
    发明授权
    Identifying pharmacophore containing combinations of scaffold molecules and substituents from a virtual library 失效
    从虚拟文库鉴定含有支架分子和取代基组合的药效团

    公开(公告)号：US06343257B1

    公开(公告)日：2002-01-29

    申请号：US09296747

    申请日：1999-04-23

    申请人： Roberto Olender ,  Rakefet Rosenfeld

    发明人： Roberto Olender ,  Rakefet Rosenfeld

    IPC分类号： G01N3348

    CPC分类号： C07K1/047

    摘要： A method for constructing a potentially pharmaceutically useful molecule that displays a predefined pharmacophore associated with a desired biological activity is disclosed. The pharmacophore is first used to screen a virtual library of scaffolds, described by three-dimensional coordinates, in order to identify one or more scaffold(s) which could potentially display substituents that alone or together with scaffold atoms comprise a pharmacophore. The screening process involves first placing rotamers from a virtual library of substituents onto each attachment point of each scaffold, and then examining the resultant Virtual Combinatorial Library (VCL) according to one or more parameters required by the pharmacophore. Preferably, the VCLs are filtered with a series of filters of increasing complexity, in order to eliminate those scaffolds incompatible with the pharmacophore of choice and to identify scaffolds which could display substituents compatible with the pharmacophore, as well the corresponding substituent rotamers themselves. The compatible scaffolds and corresponding substituents are combined to represent one or more molecule or molecules containing the desired pharmacophore. Thus, the method of the present invention is able to efficiently screen very large numbers of molecular entities for the presence of a desired pharmacophore.

    摘要翻译： 公开了一种构建显示与所需生物活性相关联的预定药效团的潜在的药学上有用的分子的方法。 药效团首先用于筛选由三维坐标描述的支架的虚拟文库，以便鉴定可能单独或与支架原子一起包含药效团的取代基的一个或多个支架。 筛选过程包括首先将来自取代基的虚拟文库的旋转异构体放置到每个支架的每个附着点上，然后根据药效团所需的一个或多个参数检查所得的虚拟组合库（VCL）。 优选地，用一系列增加复杂性的过滤器过滤VCL，以便消除与所选择的药效团不相容的那些支架，并鉴定可显示与药效团相容的取代基的支架以及相应的取代基旋转异构体本身。 将相容的支架和相应的取代基组合以代表含有所需药效团的一个或多个分子或分子。 因此，本发明的方法能够有效地筛选非常大量的分子实体来存在所需的药效团。