公开(公告)号：US07807375B2

公开(公告)日：2010-10-05

申请号：US12259376

申请日：2008-10-28

Applicant: Charles R Cantor ,  Fouad A Siddiqi

Inventor： Charles R Cantor ,  Fouad A Siddiqi

IPC: C12Q1/68

CPC classification number: C12Q1/6872 ,  C12Q2525/101 ,  C12Q2523/107

Abstract: The present invention provides a method for determining nucleic acid sequences of a template nucleic acid that requires no prior knowledge of the nucleic acid sequence present in the template nucleic acid. The method is based on combining information about the mass of a fragment, the mass of any one nucleotide and the combinations thereof, and the sequence specificity of a nucleotide cutter, either enzymatic or chemical cutter, to determine a sequence of a nucleic acid fragment. This method allows for de novo detection of sequences in a target nucleic acid without requiring any prior sequence information. This method is called Partial Sequencing by Fragmentation (PSBF) and it works by fragmenting a target into oligo- or polynucleotides whose masses or lengths are uniquely associated with known sequences. The identities of these sequences are determined solely by the specific fragmentation method used, and are always independent of the target. PSBF can be implemented using electrophoresis, mass spectrometry or any other method that can be used to distinguish the size of the cut nucleic acid sequence fragments.

Abstract translation: 本发明提供了一种确定模板核酸的核酸序列的方法，其不需要模板核酸中存在的核酸序列的先验知识。 该方法基于组合关于片段的质量，任何一个核苷酸的质量及其组合的信息以及核苷酸切割剂（酶或化学切割机）的序列特异性，以确定核酸片段的序列。 该方法允许从头检测靶核酸中的序列，而不需要任何先前的序列信息。 这种方法被称为通过片段化分段测序（PSBF），它的作用是通过将目标片段分成其质量或长度与已知序列唯一相关的寡核苷酸或多核苷酸。 这些序列的身份完全由所使用的具体碎片方法确定，并且始终与目标无关。 PSBF可以使用电泳，质谱法或可用于区分切割的核酸序列片段的大小的任何其它方法来实现。