公开(公告)号：US20240336901A1

公开(公告)日：2024-10-10

申请号：US18654176

申请日：2024-05-03

申请人： Temple University-Of The Commonwealth System of Higher Education

发明人： Richard T. Pomerantz ,  Tatiana Kent

IPC分类号： C12N9/12 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/6853

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/6853 ,  C07H21/04 ,  C12N2310/344 ,  C12Q2521/101 ,  C12Q2525/101 ,  C12Q2563/137 ,  C12Y207/07007

摘要： The invention provides compositions and methods for modifying the 3′-terminal ends of nucleic acids using DNA polymerase θ terminal transferase activity.

公开(公告)号：US11851703B2

公开(公告)日：2023-12-26

申请号：US16949159

申请日：2020-10-15

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC分类号： C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12N15/10 ,  C12Q1/6886 ,  C12Q1/6874

CPC分类号： C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q1/6855 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

摘要： The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US09994894B2

公开(公告)日：2018-06-12

申请号：US14114506

申请日：2012-04-26

申请人： Dmitry Cherkasov ,  Claus Becker ,  Norbert Basler ,  Andreas Muller-Hermann ,  Petra Van Husen

发明人： Dmitry Cherkasov ,  Claus Becker ,  Norbert Basler ,  Andreas Muller-Hermann ,  Petra Van Husen

IPC分类号： C12Q1/68 ,  C07H21/02

CPC分类号： C12Q1/686 ,  C12Q1/6846 ,  C12Q2525/101 ,  C12Q2563/149 ,  C12Q2563/179

摘要： The invention relates to a novel method for the enzymatic marking of nucleic acid chains (target sequences) by means of nucleotide conjugates. Said nucleotide conjugates are able, under reaction conditions, to specifically bind to the target sequence and integrate into the complementary growing strand by means of a polymerase. The nucleic acid chains marked by such conjugates can be bound to the solid phase. The marking can be carried out parallel to the enzymatic amplification of target sequences.

公开(公告)号：US20180148775A1

公开(公告)日：2018-05-31

申请号：US15569080

申请日：2016-04-22

申请人： ATILA BIOSYSTEMS, INC.

发明人： Youxiang Wang ,  Zhijie Yang ,  Xin Chen

IPC分类号： C12Q1/6848

CPC分类号： C12Q1/6848 ,  C12Q2525/101 ,  C12Q2531/113 ,  C12Q2537/157 ,  C12Q2537/159

摘要： The invention provides methods of amplification from a single primer or a pair of forward and reverse primers of limited nucleotide composition. Limited nucleotide composition means that the primers are underrepresented in at least one nucleotide type. Such primers have much reduced capacity to prime from each other or to extend initiated by mispriming from other than at their intended primer binding sites in a target nucleic acid.

公开(公告)号：US09868947B2

公开(公告)日：2018-01-16

申请号：US15146621

申请日：2016-05-04

申请人： Washington University

发明人： Christine Gurnett ,  Gabriel Haller ,  Matthew Dobbs ,  David Alvarado

IPC分类号： C12P19/40 ,  C12N15/10

CPC分类号： C12N15/1031 ,  C12N15/102 ,  C12N15/1058 ,  C12N15/1093 ,  C12P19/40 ,  C12Q2525/186 ,  C12Q2521/525 ,  C12Q2521/539 ,  C12Q2525/101 ,  C12Q2525/117 ,  C12Q2531/101 ,  C12Q2531/113

摘要： The present disclosure provides a method of making a systematic single point mutation in a target nucleic acid and a method of generating a mutational library comprising target nucleic acids with single point mutations. The mutational library comprises target nucleic acids with single point mutations distributed evenly throughout the target nucleic acid.

公开(公告)号：US20170159118A1

公开(公告)日：2017-06-08

申请号：US15436115

申请日：2017-02-17

申请人： Illumina, Inc.

发明人： Martin Maria Fabani ,  Maria Candelaria Rogert Bacigalupo ,  John A. Moon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  C07H21/02 ,  C07H21/04 ,  C12N9/00 ,  C12P19/30 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2521/119 ,  C12Q2525/101 ,  C12Q2533/101 ,  C12Q2533/107 ,  C12Q2535/122 ,  C12Q2565/507 ,  C12Q2565/518 ,  C12Q2565/537

摘要： A method for sequencing includes steps of (a) providing first and second nucleic acid templates, wherein the two templates have different sequences; (b) extending a first primer bound to the first template using a first polymerase species and a first set of nucleotide analogs; (c) extending a second primer bound to the second template using a second polymerase species and a second set of nucleotide analogs, wherein the first polymerase species is different from the second polymerase species and wherein the first set of nucleotide analog is different from the second set of nucleotide analog, (d) detecting the first and second primer extension products; and (e) repeating steps (b) through (d), thereby determining the different sequences of the first and second templates.

公开(公告)号：US20170114421A1

公开(公告)日：2017-04-27

申请号：US15386298

申请日：2016-12-21

申请人： LUMINEX CORPORATION

发明人： Michael James MOSER

IPC分类号： C12Q1/70

CPC分类号： C12Q1/703 ,  C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2527/107 ,  C12Q2525/161 ,  C12Q2525/101 ,  C12Q2549/119

摘要： Disclosed are methods and kits for identifying and characterizing polynucleotide sequences in a sample which may include a heterogeneous sample. Some of the methods and kits are directed to the identification and characterization of a virus in a sample, which may include HIV capable of cause AIDS or AIDS-like symptoms. The virus may be HIV-1, and may also include drug resistant mutations. The methods may include reacting a mixture that includes, in addition to nucleic acid isolated from the sample, at least one oligonucleotide capable of specifically hybridizing to HIV nucleic acid where the oligonucleotide includes at least one non-natural base.” fu addition, the methods may include detection of one or more mutations in HIV nucleic acid that are associated with drug resistance.

公开(公告)号：US09624539B2

公开(公告)日：2017-04-18

申请号：US14119846

申请日：2012-05-23

申请人： Jingyue Ju ,  Jian Wu ,  Zengmin Li

发明人： Jingyue Ju ,  Jian Wu ,  Zengmin Li

IPC分类号： C12Q1/68 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00

CPC分类号： C07H19/06 ,  C07H19/16 ,  C07H21/00 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/101 ,  C12Q2533/101 ,  C12Q2565/632

摘要： This invention provides nucleoside triphosphate analogs having the structure: wherein B is a base and is adenine, guanine, cytosine, uracil or thymine, wherein R″ is an OH or an H, and wherein R′ is azidomethyl, a hydrocarbyl, or a substituted hydrocarbyl, and which has a Raman spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1 or a Fourier transform-infrared spectroscopy spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1, and also to methods of DNA sequencing and SNP detection.

公开(公告)号：US20170073740A1

公开(公告)日：2017-03-16

申请号：US15311149

申请日：2015-05-14

申请人： Stratos Genomics, Inc.

发明人： Robert N. McRuer ,  Mark Stamatios Kokoris

IPC分类号： C12Q1/68 ,  G01N27/447 ,  G01N33/487

CPC分类号： C12Q1/6825 ,  C12Q1/6869 ,  C12Q2523/307 ,  C12Q2525/101 ,  C12Q2525/197 ,  C12Q2525/301 ,  C12Q2565/631 ,  G01N27/44704 ,  G01N27/44791 ,  G01N33/48721

摘要： Translocation control for sensing by a nanopore, as well as methods and products related to the same, are provided. Such methods optimize duplex stability to provide high fill rate (of the hybridization sites) but do not prevent rapid dissociation required for high read rates, as well as controlling the translocation of a target molecule for sensing by a nanopore by use of a selective pulsed voltage. Products related to the same include a reporter construct comprising two or more phosphoramidites.

摘要翻译： 提供了由纳米孔感测的易位控制，以及与其相关的方法和产品。 这样的方法优化双重稳定性以提供高填充率（杂交位点），但是不能防止高读取速率所需的快速解离，以及通过使用选择性脉冲电压来控制靶分子易于被纳米孔感测的易位 。 与其相关的产品包括包含两个或多个亚磷酰胺的报道构建体。

公开(公告)号：US09441272B2

公开(公告)日：2016-09-13

申请号：US14299254

申请日：2014-06-09

申请人： Illumina Cambridge Limited

发明人： Roberto Rigatti ,  Jonathan Mark Boutell ,  Jason Richard Betley ,  Niall Anthony Gormley

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q2525/101 ,  C12Q2525/186 ,  C12Q2525/191 ,  C12Q2565/543 ,  C12Q2565/537 ,  C12Q2535/122 ,  C12Q2523/301 ,  C12Q2521/319

摘要： Disclosed herein are compositions and methods for sequencing nucleic acids.

摘要翻译： 本文公开了用于测序核酸的组合物和方法。