公开(公告)号：US20190031704A1

公开(公告)日：2019-01-31

申请号：US16149098

申请日：2018-10-01

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C07H19/14 ,  C12Q1/6869 ,  C07H19/10 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6872 ,  C12Q1/68 ,  C12Q1/686

CPC分类号： C12Q1/6869 ,  C07B2200/11 ,  C07H19/10 ,  C07H19/14 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2525/186 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2565/501 ,  C40B40/00

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US20180208980A1

公开(公告)日：2018-07-26

申请号：US15900308

申请日：2018-02-20

申请人： Omniome, Inc.

发明人： Corey M. Dambacher ,  Michael Van Nguyen

IPC分类号： C12Q1/6853 ,  C12Q1/6883 ,  C12Q1/6872 ,  C12Q1/686

CPC分类号： C12Q1/6853 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2522/101 ,  C12Q2565/501

摘要： A method for identifying target alleles, that includes steps of (a) forming a plurality of stabilized ternary complexes at a plurality of features on an array, wherein the stabilized ternary complexes each has a polymerase, a template nucleic acid having a target allele of a locus, a primer hybridized to the locus, and a next correct nucleotide having a cognate in the locus, wherein either (i) the primer is an allele-specific primer having a 3′ nucleotide that is a cognate nucleotide for the target allele, or (ii) the primer is a locus-specific primer and the next correct nucleotide hybridizes to the target allele; and (b) detecting stabilized ternary complexes at the features, thereby identifying the target alleles.

公开(公告)号：US20180030531A1

公开(公告)日：2018-02-01

申请号：US15663528

申请日：2017-07-28

申请人： The Regents of the University of California

发明人： Markus de Raad ,  Trent R. Northen ,  Curt R. Fischer

IPC分类号： C12Q1/68 ,  C12Q1/25 ,  G01N33/573 ,  C12N15/10

CPC分类号： C12Q1/6872 ,  C12N15/10 ,  C12P21/06 ,  C12Q1/25 ,  G01N33/573

摘要： Disclosed herein are methods, systems and compositions for determining substrate specificity of an enzyme. The disclosed methods, systems and compositions can be used for identifying enzymes capable of modifying substrates of interest and/or quantifying enzymatic activity.

公开(公告)号：US20170369857A1

公开(公告)日：2017-12-28

申请号：US15690945

申请日：2017-08-30

申请人： Life Technologies Corporation

发明人： Peter VANDER HORN ,  Cheng-Yao CHEN ,  Guobin LUO ,  Michael PREVITE ,  Jamshid TEMIROV ,  Theo NIKIFOROV ,  Zhaohui ZHOU ,  Hongye SUN ,  Yufang WANG ,  Stefanie Yukiko NISHIMURA ,  Hongyi WANG ,  Marian PERIS ,  Barnett ROSENBLUM ,  Michael PHELAN

IPC分类号： C12N9/12 ,  C12Q1/68

CPC分类号： C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

摘要： Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US20170283789A1

公开(公告)日：2017-10-05

申请号：US15507218

申请日：2015-08-27

申请人： HITACHI CHEMICAL CO., LTD. ,  HITACHI CHEMICAL CO. AMERICA, LTD.

发明人： Cindy M. Yamamoto ,  Vivian Tovar ,  Taku Murakami ,  Mieko Ogura ,  Melanie Oakes ,  Masato Mitsuhashi

IPC分类号： C12N15/10 ,  C12Q1/68

CPC分类号： C12N15/1003 ,  C12N15/1072 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158

摘要： The present disclosure relates to methods of collecting cervical-vaginal fluid exosomes and microvesicles and isolating corresponding mRNA. In particular, certain embodiments relate to the method of collecting the cervical-vaginal fluids with a tampon and releasing the cells, exosomes and microvesicles using excess buffer and a syringe or syringe-like device. The resulting expunged fluid can be applied to a filter device that is capable of capturing exosomes and microvesicles. Nucleic acids such as mRNA can be isolated from the cervical-vaginal fluid exosome and microvesicles using an oligo(dT)-coated plate designed to accommodate the filter device and then used for further molecular analysis. Quantification of the collected nucleic acids may then be used in the diagnosis and/or treatment of gynecological diseases or conditions.

公开(公告)号：US20170175092A1

公开(公告)日：2017-06-22

申请号：US15422224

申请日：2017-02-01

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Marian PERIS ,  Michael PHELAN ,  Barnett ROSENBLUM ,  Stephen HENDRICKS

IPC分类号： C12N9/12

CPC分类号： C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

摘要： Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

公开(公告)号：US20170088891A1

公开(公告)日：2017-03-30

申请号：US15380311

申请日：2016-12-15

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C12Q1/68 ,  C07H19/10

CPC分类号： C07H19/14 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C40B40/00 ,  C12Q2565/501 ,  C12Q2525/186

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US09541562B2

公开(公告)日：2017-01-10

申请号：US14739864

申请日：2015-06-15

申请人： Quest Diagnostics Investments Incorporated

发明人： Amit Ghoshal ,  Nigel J. Clarke ,  Mildred M. Goldman

IPC分类号： G01N33/00 ,  H01J49/04 ,  G01N33/74 ,  G01N33/68 ,  H01J49/00 ,  C12Q1/68

CPC分类号： G01N33/743 ,  C12Q1/6872 ,  G01N30/7233 ,  G01N33/6848 ,  G01N33/6851 ,  G01N33/689 ,  G01N2800/04 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/165

摘要： Methods are provided for detecting the amount of one or more CAH panel analytes (i.e., pregnenolone, 17-OH pregnenolone, progesterone, 17-OH progesterone, dehydroepiandrosterone (DHEA), androstenedione, testosterone, deoxycorticosterone, 11-deoxycortisol, and cortisol) in a sample by mass spectrometry. The methods generally involve ionizing one or more CAH panel analytes in a sample and quantifying the generated ions to determine the amount of one or more CAH panel analytes in the sample. In methods where amounts of multiple CAH panel analytes are detected, the amounts of multiple analytes are detected in the same sample injection.

摘要翻译： 提供了用于检测一种或多种CAH组分析物（即孕烯醇酮，17-OH孕烯醇酮，孕酮，17-OH孕酮，脱氢表雄酮（DHEA），雄烯二酮，睾酮，脱氧皮质酮，11-脱氧皮质醇和皮质醇）的量的方法 质谱样品。 所述方法通常涉及将样品中的一种或多种CAH面板分析物离子化并量化产生的离子以确定样品中一种或多种CAH面板分析物的量。 在检测到多个CAH面板分析物的量的方法中，在相同的样品注入中检测到多种分析物的量。

公开(公告)号：US09416424B2

公开(公告)日：2016-08-16

申请号：US13174254

申请日：2011-06-30

申请人： David J. Ecker ,  Richard H. Griffey ,  Rangarajan Sampath ,  Steven A. Hofstadler ,  John McNeil ,  Stanley T. Crooke ,  Lawrence Blyn ,  Ray Ranken ,  Thomas A. Hall

发明人： David J. Ecker ,  Richard H. Griffey ,  Rangarajan Sampath ,  Steven A. Hofstadler ,  John McNeil ,  Stanley T. Crooke ,  Lawrence Blyn ,  Ray Ranken ,  Thomas A. Hall

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12Q1/70 ,  G01N1/30 ,  G01N21/64 ,  G01N23/00 ,  G06F19/20

CPC分类号： C12Q1/689 ,  C12Q1/6872 ,  C12Q1/70 ,  C12Q1/701 ,  C12Q2600/16 ,  G01N1/30 ,  G01N21/64 ,  G01N23/00 ,  G06F19/20

摘要： The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples.

摘要翻译： 本发明提供了以下方法：鉴定来自人和动物的生物样品中的病原体，解决从人和动物获得的样品中存在的多种病原体，确定关于这种病原体或病原体的详细遗传信息，以及快速检测和鉴定生物代谢 来自环境，临床或其他样品。

公开(公告)号：US20160104610A1

公开(公告)日：2016-04-14

申请号：US14886979

申请日：2015-10-19

申请人： IBIS BIOSCIENCES, INC.

发明人： Steven A. Hofstadler ,  Jose R. Gutierrez ,  James C. Hannis ,  Jared J. Drader ,  Rex O. Bare ,  Jeffrey C. Smith ,  Paul J. Gleason ,  Jared Nathanson

IPC分类号： H01J49/16 ,  H01J49/04

CPC分类号： H01J49/165 ,  C12Q1/6872 ,  G01N23/00 ,  G01R31/2812 ,  G01R31/2848 ,  G01R31/2853 ,  H01J27/02 ,  H01J49/0422 ,  H01J49/0431 ,  H01J49/0468

摘要： The invention relates generally to sample ionization, and provides ionization probe assemblies, systems, computer program products, and methods useful for this purpose.