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公开(公告)号：US07790188B2
公开(公告)日：2010-09-07
申请号：US11646352
申请日：2006-12-27
申请人： Ching-Yu Tu , Li-Ling Liaw
发明人： Ching-Yu Tu , Li-Ling Liaw
CPC分类号： C12N15/8282 , A01N63/04 , A61K38/00 , C07K14/37
摘要： The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.
摘要翻译： 本发明涉及通过开发红曲霉BCRC 38072的全基因组序列和单基因数据库获得的抗真菌蛋白质基因及其cDNA序列。 该基因可编码抗真菌蛋白MAFP1。 通过N末端蛋白质测序和比较分析，将得自具有约7kDa分子量的M. pilosus培养液的纯化蛋白质鉴定为MAFP1。 纯化的MAFP1蛋白可以抑制病原体的生长，如拟青霉BCPC 33174和Helminthosporium panici BCRC 35004.另外，通过PCR检测发现，该抗真菌蛋白的基因存在于其他红曲霉属物种如M.Barkeri，M 花椒（M.orisporas），马尾松属（M. pilosus），木霉属（M.ruber）等。 也证明了四种红曲霉菌（M.closlosus）（BCRC 38072，BCRC 38093和BCRC 31502）和M.ruber BCRC 31533中的mafp1基因及其cDNA具有相同的DNA序列。
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公开(公告)号：US20080161225A1
公开(公告)日：2008-07-03
申请号：US11646352
申请日：2006-12-27
申请人： Ching-Yu Tu , Li-Ling Liaw
发明人： Ching-Yu Tu , Li-Ling Liaw
IPC分类号： A01H5/00 , A01N37/18 , A23J1/18 , A61K38/00 , A61P43/00 , C07H21/04 , C12N15/00 , C12N5/04 , C12Q1/68
CPC分类号： C12N15/8282 , A01N63/04 , A61K38/00 , C07K14/37
摘要： The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.
摘要翻译： 本发明涉及通过开发红曲霉BCRC 38072的全基因组序列和单基因数据库获得的抗真菌蛋白质基因及其cDNA序列。 该基因可编码抗真菌蛋白MAFP1。 通过N末端蛋白质测序和比较分析，将得自具有约7kDa分子量的M. pilosus培养液的纯化蛋白质鉴定为MAFP1。 纯化的MAFP1蛋白可以抑制病原体的生长，如拟青霉BCPC 33174和Helminthosporium panici BCRC 35004.另外，通过PCR检测发现，该抗真菌蛋白的基因存在于其他红曲霉属物种如M.Barkeri，M 花椒（M.orisporas），马尾松属（M. pilosus），木霉属（M.ruber）等。 也证明了四种红曲霉菌（M.closlosus）（BCRC 38072，BCRC 38093和BCRC 31502）和M.ruber BCRC 31533中的mafp1基因及其cDNA具有相同的DNA序列。
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