公开(公告)号：US20220145342A1

公开(公告)日：2022-05-12

申请号：US17434967

申请日：2020-02-28

申请人： Chr Hansen HMO GmbH

发明人： Stefan JENNEWEIN ,  Dirk WARTENBERG

IPC分类号： C12P19/02 ,  C12P19/04 ,  C12P19/18

摘要： Disclosed are genetically engineered microbial cells for the production of a carbohydrate of interest, wherein the microbial cells possess an increased intracellular availability of at least one sugar phosphate, and produce the carbohydrate of interest when being cultivated in a culture medium containing a mixed monosaccharide feedstock as main carbon and energy source. Also disclosed is a fermentative method of producing a carbohydrate of interest by cultivating said genetically engineered microbial cell in the presence of a mixed monosaccharide feedstock as main carbon and energy source.

公开(公告)号：US20220220524A1

公开(公告)日：2022-07-14

申请号：US17618254

申请日：2020-06-08

申请人： Chr Hansen HMO GmbH

发明人： Stefan JENNEWEIN ,  Dirk WARTENBERG ,  Stefanie HABERSETZER

IPC分类号： C12P19/18 ,  C12N15/52 ,  C12N9/10 ,  C12N9/12 ,  C12N9/24

摘要： Disclosed are non-sporulating Bacillus cells for the production of a fucosylated oligosaccharide as well as a method for producing a fucosylated oligosaccharide, wherein said Bacillus cell has been genetically engineered to possess a lactose permease, a GDP-fucose biosynthesis pathway and a fucosyltransferase.