公开(公告)号：US20240228944A9

公开(公告)日：2024-07-11

申请号：US18254658

申请日：2021-11-29

Applicant: Chr. Hansen A/S

Inventor： Zuzana Mladenovska ,  Surender Kumar Dhayal ,  Kim Nielsen

IPC: C12N1/04 ,  C12N1/20

CPC classification number: C12N1/04 ,  C12N1/20

Abstract: The present invention relates to the field of frozen or dry compositions for prokaryotes, in particular fermentative bacteria such as lactic acid bacteria, a method for preparing frozen or dry prokaryotic compositions and compositions which may be prepared by said method. The cells in a cell concentrate are activated by allowing them to ferment a carbohydrate, before freezing and/or drying to provide a product. The hydrophobicity of the cell surface increases during activation, and this has been found to increase the storage stability of the product.

公开(公告)号：US20240141282A1

公开(公告)日：2024-05-02

申请号：US17799190

申请日：2021-02-25

Applicant: Chr. Hansen A/S

Inventor： Michelle Milling Madsen ,  Kim Nielsen ,  Jimi Kjaersgaard Pettersson ,  Wendy Ossieur

IPC: C12N1/04 ,  C12N1/20

CPC classification number: C12N1/04 ,  C12N1/20

Abstract: The present invention provides a process for spray freezing or spray freeze drying under conditions of very low oxygen pressure, such as under essentially anaerobic conditions, and particularly a process for spray freezing strict anaerobic microorganisms (bacteria) under conditions where the level of oxygen is very low, or essentially anaerobic. Further, the invention provides a product achievable by the processes and an apparatus usable in the process.

公开(公告)号：US20230341182A1

公开(公告)日：2023-10-26

申请号：US17795211

申请日：2021-01-29

Applicant: Chr. Hansen A/S

Inventor： Jimi Kjaersgaard Pettersson ,  Wendy Ossieur ,  Michelle Milling Madsen ,  Kim Nielsen

IPC: C12M1/42 ,  C12M1/00 ,  F26B3/12 ,  C12N13/00

CPC classification number: F26B3/12 ,  C12M35/02 ,  C12M47/14 ,  C12N13/00

Abstract: The present disclosure relates to a process for electrostatic spray drying of a living microorganism, the process comprising the following steps: a. Providing a suspension, comprising a number of components, including a microorganism, a solvent and an additive; b. Applying an electrostatic charge to said suspension; c. Forming droplets of said suspension; d. Drying said droplets, thereby forming dried particles; and e. (Optionally) collecting the dried particles.

公开(公告)号：US20240132832A1

公开(公告)日：2024-04-25

申请号：US18254658

申请日：2021-11-29

Applicant: Chr. Hansen A/S

Inventor： Zuzana Mladenovska ,  Surender Kumar Dhayal ,  Kim Nielsen

IPC: C12N1/04 ,  C12N1/20

CPC classification number: C12N1/04 ,  C12N1/20

Abstract: The present invention relates to the field of frozen or dry compositions for prokaryotes, in particular fermentative bacteria such as lactic acid bacteria, a method for preparing frozen or dry prokaryotic compositions and compositions which may be prepared by said method. The cells in a cell concentrate are activated by allowing them to ferment a carbohydrate, before freezing and/or drying to provide a product. The hydrophobicity of the cell surface increases during activation, and this has been found to increase the storage stability of the product.