公开(公告)号：US07129053B1

公开(公告)日：2006-10-31

申请号：US10110410

申请日：2000-10-12

申请人： Christian Reiter ,  Gerhard Cullman ,  Meret Lakner ,  Andreas Trüe ,  Sonja Dehnert ,  Georg Schwartz

发明人： Christian Reiter ,  Gerhard Cullman ,  Meret Lakner ,  Andreas Trüe ,  Sonja Dehnert ,  Georg Schwartz

IPC分类号： G01N1/30 ,  G01N33/53 ,  G01N33/567 ,  G01N33/569

CPC分类号： C07K16/121 ,  C07K16/40 ,  C07K2317/565 ,  G01N33/558 ,  G01N33/56911 ,  G01N33/56922 ,  G01N33/5695 ,  G01N33/573 ,  G01N2333/908 ,  G01N2333/96486 ,  G01N2333/98 ,  Y10S435/975

摘要： The invention relates to a method for detecting an infection of a mammal with an acid-resistant microorganism comprising the following steps: (a) provision of an immunochromatographic test with a sample application area for the application of a stool sample of the mammal with an antigen and application of the stool sample, (b) incubation of the stool sample using (i) a first receptor under conditions permitting a complex formation of the antigen from the acid resistant microorganism with the receptor; or (ii) at least two different first receptors under conditions permitting a complex formation of the antigen from the acid-resistant microorganism with the at least two first receptors and wherein the first receptor according to (i) or the first receptors according to (ii) specifically bind(s) an antigen which shows, at least with some mammals, a structure after passage through the intestine that corresponds to the native structure or the structure against which a mammal produces antibodies against after being infected or immunized with the acid-resistant microorganism or an extract or lysate thereof or a protein therefrom or a fragment thereof or a synthetic peptide produces antibodies; and (c) provision of a second receptor immobilized at an analysis region, wherein the second receptor binds an antigen receptor complex according to (b) and (d) transport and detection of the formation of at least one antigen receptor complex according to (b) by accumulation of the antigen receptor complex at the second receptor in the analysis area. The invention further relates to an immunochromatographic test which is particularly suitable and designed to carry out the method of the invention.

摘要翻译： 本发明涉及一种用耐酸微生物检测哺乳动物感染的方法，包括以下步骤：（a）提供具有样品施用区域的免疫层析试验，以用于将哺乳动物的粪便样品与抗原 和粪便样品的施用，（b）使用（i）第一受体在允许来自抗酸微生物的抗原与受体复合形成的条件下温育粪便样品; 或（ii）至少两种不同的第一受体，其条件是允许具有至少两个第一受体的抗酸微生物的抗原复合形成，并且其中根据（i）的第一受体或根据（ii）的第一受体 ）特异性结合至少与一些哺乳动物通过肠道的结构，该结构对应于天然结构或结构，其中哺乳动物产生针对感染后的抗体或用耐酸性免疫的结构 微生物或其提取物或裂解物或其蛋白质或其片段或合成肽产生抗体; （c）提供固定在分析区域的第二受体，其中所述第二受体根据（b）和（d）结合抗原受体复合物，根据（b）的转运和检测至少一种抗原受体复合物的形成 ）通过在分析区域中的第二受体处的抗原受体复合物的积累。 本发明还涉及一种特别适合和设计用于实施本发明方法的免疫层析试验。