公开(公告)号：US07076092B2

公开(公告)日：2006-07-11

申请号：US10170876

申请日：2002-06-12

申请人： Christopher W. Hollars ,  Thomas R. Huser ,  Stephen M. Lane ,  Rodney L. Balhorn ,  Olgica Bakajin ,  Christopher Darrow ,  Joe H. Satcher, Jr.

发明人： Christopher W. Hollars ,  Thomas R. Huser ,  Stephen M. Lane ,  Rodney L. Balhorn ,  Olgica Bakajin ,  Christopher Darrow ,  Joe H. Satcher, Jr.

IPC分类号： G06K9/00

CPC分类号： G06K9/00127 ,  B01L3/5027 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/54353 ,  G01N33/582 ,  G01N2021/058 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6441

摘要： A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0.5 μm×100 μm×100 μm. A device using these methods would have 103–104 greater throughput than previous one-dimensional (1D) micro-stream devices having 1 μm3 interrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (˜10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.

摘要翻译： 通过双重荧光标记的探针分子的定位，灵敏度检测和识别单个靶分子的方法和装置。 这可以通过将标签特定附着到具有约0.5mum×100mum×100mum的大小的表面或二维（2D）流动流体片材中来实现。 使用这些方法的装置将具有比具有1mum 3以上的一维（1D）微流装置更高的产量10％以上。 >询问体积，并将首次允许在短时间（〜10分钟）内进行超低（飞摩尔）浓度的免疫和DNA测定。 可以引入新的标记（例如金属或半导体纳米颗粒）的使用以进一步将灵敏度扩展到极限范围内。

公开(公告)号：US07301624B2

公开(公告)日：2007-11-27

申请号：US10935783

申请日：2004-09-07

申请人： Chad E. Talley ,  Thomas R. Huser ,  Christopher W. Hollars ,  Stephen M. Lane ,  Joe H. Satcher, Jr. ,  Bradley R. Hart ,  Ted A. Laurence

发明人： Chad E. Talley ,  Thomas R. Huser ,  Christopher W. Hollars ,  Stephen M. Lane ,  Joe H. Satcher, Jr. ,  Bradley R. Hart ,  Ted A. Laurence

IPC分类号： G01J3/44

CPC分类号： G01N21/658 ,  G01J3/44

摘要： Surface-Enhanced Raman Spectroscopy (SERS) is a vibrational spectroscopic technique that utilizes metal surfaces to provide enhanced signals of several orders of magnitude. When molecules of interest are attached to designed metal nanoparticles, a SERS signal is attainable with single molecule detection limits. This provides an ultrasensitive means of detecting the presence of molecules. By using selective chemistries, metal nanoparticles can be functionalized to provide a unique signal upon analyte binding. Moreover, by using measurement techniques, such as, ratiometric received SERS spectra, such metal nanoparticles can be used to monitor dynamic processes in addition to static binding events. Accordingly, such nanoparticles can be used as nanosensors for a wide range of chemicals in fluid, gaseous and solid form, environmental sensors for pH, ion concentration, temperature, etc., and biological sensors for proteins, DNA, RNA, etc.

摘要翻译： 表面增强拉曼光谱（SERS）是一种振动光谱技术，利用金属表面提供几个数量级的增强信号。 当感兴趣的分子连接到设计的金属纳米粒子时，可以通过单分子检测限达到SERS信号。 这提供了检测分子存在的超灵敏手段。 通过使用选择性化学成分，金属纳米粒子可以被功能化，以在分析物结合时提供独特的信号。 此外，通过使用诸如比例接收的SERS光谱的测量技术，除了静态结合事件之外，这种金属纳米颗粒可用于监测动态过程。 因此，这种纳米颗粒可用作流体，气体和固体形式的广泛化学品的纳米传感器，用于pH，离子浓度，温度等的环境传感器，以及用于蛋白质，DNA，RNA等的生物传感器。

公开(公告)号：US20080239307A1

公开(公告)日：2008-10-02

申请号：US11731418

申请日：2007-03-30

申请人： Chad E. Talley ,  Thomas R. Huser ,  Christopher W. Hollars ,  Stephen M. Lane

发明人： Chad E. Talley ,  Thomas R. Huser ,  Christopher W. Hollars ,  Stephen M. Lane

IPC分类号： G01J3/44

CPC分类号： G01J3/44 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/0262 ,  G01N21/658

摘要： A surface-enhanced Raman scattering method and apparatus to sequence polymeric biomolecules such as DNA, RNA, or proteins is introduced. The method uses metallic nanostructures such as, for example, spherical or cylindrical Au or Ag nanoparticles having characteristic lengths of 10-100 nm which when illuminated with light of the appropriate wavelength produce resonant oscillations of the conduction electrons (plasmon resonance). Electric field enhancements of 30-1000 near the particle surface resulting from such oscillations increase Raman scattering cross-sections by about 106-1015 due to the E4 dependence of the Raman scattering, wherein the largest enhancements occur in the gap/junction between novel closely spaced structures as disclosed herein.

摘要翻译： 引入表面增强拉曼散射方法和用于对聚合物生物分子如DNA，RNA或蛋白质进行排序的装置。 该方法使用金属纳米结构，例如具有特征长度为10-100nm的球形或圆柱形Au或Ag纳米颗粒，当用适当波长的光照射时，会导致传导电子（等离子体共振）的共振振荡。 由于这种振荡导致的颗粒表面附近的30-1000的电场增强使由于E 4引起的拉曼散射横截面增加约10 -6 -10 15 拉曼散射的依赖性，其中最大的增强发生在本文公开的新颖的紧密间隔的结构之间的间隙/接合处。