公开(公告)号：US20040127648A1

公开(公告)日：2004-07-01

申请号：US10334135

申请日：2002-12-31

Applicant: Ciphergen Biosystems, Inc.

Inventor： Luc Guerrier ,  Egisto Boschetti

IPC: C08L033/06

CPC classification number: C12N15/101 ,  C08L33/14 ,  C08L33/24 ,  C08L51/003 ,  C08L53/00 ,  C12N15/1006 ,  C08L2666/04 ,  C08L2666/02

Abstract: A polymer that is useful for separating plasmid DNA from cell lysates can be dispersed throughout the pores of a porous matrix, forming a chromatographic sorbent. Illustrative of such a polymer is one that comports with the formula: 1 where R1 is C1-6 alkyl; each R2 is independently selected from the group consisting of H and C1-6 alkyl, optionally substituted with 1 to 3 OH groups; R3 is selected from H, C1-14 alkyl, and (C1-14 alkyl)aryl; each n is independently an integer from 1 to 6; a is at least 1; b is at least 1; w is an integer from 0 to 10; and z is at least 1. X is an anion; each Y is independently selected from the group consisting of H, C1-6 alkyl, and a linear homopolymer comprised of monomers, each of them substituted with pendant amines, provided that at least one Y is the linear homopolymer; and null-----null represents the remainder of the polymer.

Abstract translation: 可用于从细胞裂解物分离质粒DNA的聚合物可分散在多孔基质的整个孔中，形成色谱吸附剂。 这样的聚合物的例子是与下式相符的聚合物：其中R 1是C 1-6烷基; 每个R 2独立地选自H和C 1-6烷基，任选被1至3个OH基团取代; R 3选自H，C 1-4烷基和（C 1-4烷基）芳基; 每个n独立地为1至6的整数; a至少为1; b至少为1; w为0〜10的整数; z为至少1. X为阴离子; 每个Y独立地选自H，C 1-6烷基和由单体组成的线性均聚物，它们各自被侧胺取代，条件是至少一个Y是线性均聚物; 和“-----”表示聚合物的其余部分。