公开(公告)号：US20030125529A1

公开(公告)日：2003-07-03

申请号：US10305399

申请日：2002-11-27

Applicant: Ciphergen Biosystems, Inc.

Inventor： Egisto Boschetti ,  Pierre Girot

IPC: C07K001/16 ,  C07H021/04

CPC classification number: B01J20/282 ,  B01J20/0211 ,  B01J20/0292 ,  B01J20/0296 ,  B01J20/048 ,  B01J20/06 ,  B01J20/08 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28078 ,  B01J20/28085 ,  B01J20/28097 ,  B01J20/286 ,  B01J20/3028 ,  B01J20/3064 ,  B01J20/3071 ,  B01J20/3078 ,  B01J20/3204 ,  B01J20/3234 ,  B01J20/3236 ,  B01J20/3242 ,  B01J20/3268 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3282 ,  B01J20/3293 ,  B01J2220/42 ,  B01J2220/52 ,  B01J2220/58 ,  C07K1/20

Abstract: A new adsorbent of a porous mineral oxide material with apatite crystals, preferably hydroxyapatite crystals, in the pores of the mineral oxide material is disclosed. The adsorbent is useful for protein and nucleic acid separations

Abstract translation: 公开了一种在矿物氧化物材料的孔中具有磷灰石晶体，优选羟基磷灰石晶体的多孔矿物氧化物材料的新型吸附剂。 吸附剂可用于蛋白质和核酸分离

公开(公告)号：US20040146937A1

公开(公告)日：2004-07-29

申请号：US10626493

申请日：2003-07-23

Applicant: Ciphergen Biosystems, Inc.

Inventor： William E. Rich ,  Egisto Boschetti ,  Lee O. Lomas ,  Tai-Tung Yip

IPC: G01N033/53

CPC classification number: C40B30/04 ,  G01N33/54306 ,  G01N33/6803

Abstract: This invention provides methods and materials for mapping interaction characteristics between components of a multicomponent biological complex. The methods involve capturing a multicomponent complex on a solid support and washing the support with a series of elution washes forming a gradient of solute concentrations, and determining whether a particular elution wash eluted a particular component.

Abstract translation: 本发明提供了用于映射多组分生物复合物的组分之间的相互作用特征的方法和材料。 该方法包括在固体支持物上捕获多组分复合物，并用洗脱洗涤洗涤洗脱洗涤洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱特定组分。

公开(公告)号：US20030218130A1

公开(公告)日：2003-11-27

申请号：US10412679

申请日：2003-04-14

Applicant: Ciphergen Biosystems, Inc.

Inventor： Egisto Boschetti ,  Jian Ding ,  Pierre Girot ,  Luc Gurrier ,  Christopher A. Pohl ,  Michael T. Grimes

IPC: H01J049/00 ,  B01D059/44

CPC classification number: G01N33/6848 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00454 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00707 ,  B01J2219/00711 ,  B01J2219/00716 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/00734 ,  B82Y30/00 ,  C08B11/20 ,  C08B15/00 ,  C08B31/00 ,  C08B33/00 ,  C08B37/0021 ,  C08B37/0039 ,  C08L5/02 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B40/14 ,  C40B50/14 ,  C40B60/14 ,  G01N33/5302 ,  G01N33/559 ,  G01N33/6851 ,  H01J49/0418

Abstract: The present invention provides a substrate having a polymerized, polysaccharide-based hydrogel attached to the surface. The hydrogel can be derivatized with binding functionalities that bind analytes from a sample. The invention further provides methods of using the device and gels that are capable of selectively binding one or more analytes from a sample.

Abstract translation: 本发明提供了具有附着在表面上的聚合的多糖基水凝胶的基材。 水凝胶可以用结合来自样品的分析物的结合官能团衍生化。 本发明还提供使用能够选择性地结合一种或多种来自样品的分析物的装置和凝胶的方法。

公开(公告)号：US20040127648A1

公开(公告)日：2004-07-01

申请号：US10334135

申请日：2002-12-31

Applicant: Ciphergen Biosystems, Inc.

Inventor： Luc Guerrier ,  Egisto Boschetti

IPC: C08L033/06

CPC classification number: C12N15/101 ,  C08L33/14 ,  C08L33/24 ,  C08L51/003 ,  C08L53/00 ,  C12N15/1006 ,  C08L2666/04 ,  C08L2666/02

Abstract: A polymer that is useful for separating plasmid DNA from cell lysates can be dispersed throughout the pores of a porous matrix, forming a chromatographic sorbent. Illustrative of such a polymer is one that comports with the formula: 1 where R1 is C1-6 alkyl; each R2 is independently selected from the group consisting of H and C1-6 alkyl, optionally substituted with 1 to 3 OH groups; R3 is selected from H, C1-14 alkyl, and (C1-14 alkyl)aryl; each n is independently an integer from 1 to 6; a is at least 1; b is at least 1; w is an integer from 0 to 10; and z is at least 1. X is an anion; each Y is independently selected from the group consisting of H, C1-6 alkyl, and a linear homopolymer comprised of monomers, each of them substituted with pendant amines, provided that at least one Y is the linear homopolymer; and null-----null represents the remainder of the polymer.

Abstract translation: 可用于从细胞裂解物分离质粒DNA的聚合物可分散在多孔基质的整个孔中，形成色谱吸附剂。 这样的聚合物的例子是与下式相符的聚合物：其中R 1是C 1-6烷基; 每个R 2独立地选自H和C 1-6烷基，任选被1至3个OH基团取代; R 3选自H，C 1-4烷基和（C 1-4烷基）芳基; 每个n独立地为1至6的整数; a至少为1; b至少为1; w为0〜10的整数; z为至少1. X为阴离子; 每个Y独立地选自H，C 1-6烷基和由单体组成的线性均聚物，它们各自被侧胺取代，条件是至少一个Y是线性均聚物; 和“-----”表示聚合物的其余部分。

公开(公告)号：US20040124149A1

公开(公告)日：2004-07-01

申请号：US10660738

申请日：2003-09-12

Applicant: Ciphergen Biosystems, Inc.

Inventor： Egisto Boschetti ,  Pierre Girot ,  Pil-Je Um

IPC: B01J020/22 ,  B01D015/08

CPC classification number: B01J20/3255 ,  B01D15/1807 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3285 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01J2219/0074 ,  C07K1/165 ,  C07K1/22 ,  C40B40/10 ,  C40B80/00 ,  G01N33/54353 ,  G01N2001/045 ,  G01N2035/00158 ,  Y10S530/812 ,  Y10T436/25

Abstract: For certain mixed mode resins having anionic character, a ligand is joined to a solid support via a linkage that includes a mercapto-, ether- or amino-containing moiety. A suitable ligand comprises an aromatic group, a heteroaromatic group, or a heterocyclic group, optionally fused, that is sulfate-, sulfonate-, phosphonate- or phosphate-substituted and that is linked to such a moiety. These resins possess an anionic character under conditions prescribed for their use. Separation of a biological substance, such as a peptide or protein, can be accomplished with a resin of this type via a change in the pH of eluants, thereby effecting adsorption and desorption.

Abstract translation: 对于具有阴离子特性的某些混合模式树脂，配体通过包含巯基，醚或氨基部分的键连接到固体支持物上。 合适的配体包括芳族基团，杂芳族基团或杂环基团，任选地稠合，其是硫酸酯基，磺酸酯基，膦酸酯基或磷酸酯取代的，并且与该部分连接。 这些树脂在其使用条件下具有阴离子特性。 生物物质如肽或蛋白质的分离可以通过这种类型的树脂通过洗脱液的pH的变化来实现，从而进行吸附和解吸附。

公开(公告)号：US20030091976A1

公开(公告)日：2003-05-15

申请号：US10124626

申请日：2002-04-16

Applicant: Ciphergen Biosystems, Inc.

Inventor： Egisto Boschetti ,  Lisa Bradbury ,  Huw Davies ,  Lee O. Lomas ,  Thang T. Pham ,  Vanitha Thulasiraman ,  Tai-Tung Yip

IPC: C12Q001/00 ,  G01N033/53 ,  G01N033/567

CPC classification number: G01N33/6842 ,  C07K1/16 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  G01N33/6803 ,  G01N2030/8411 ,  H01J49/00 ,  H01J49/0418 ,  G01N30/7233

Abstract: The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken from multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.

Abstract translation: 本发明提供了通过产生从多个细胞培养物批次或从给定批次的不同时间采集的样品获取的样品的表面增强的激光解吸/电离质谱图来监测目标多肽的产生的方法。 本发明另外提供了通过产生在纯化过程中的不同时间采集的样品的表面增强的激光解吸/电离质谱图来监测来自混合物的目标多肽的纯化的方法。 此外，本发明涉及鉴定可用于增加给定纯化方法的规模的条件的方法。