公开(公告)号：US11225682B2

公开(公告)日：2022-01-18

申请号：US15767481

申请日：2016-10-12

申请人： CureVac AG

发明人： Isabel Reichert ,  Fabian Johannes Eber ,  Christian Mayer ,  Aniela Wochner

IPC分类号： C12Q1/20 ,  C12Q1/24 ,  C12N1/20

摘要： The present invention is concerned with an automated method for obtaining at least one discrete colony from microorganisms or cells comprised in a solution. This method includes a step where acoustic liquid transfer is employed. The present invention further relates to the use of an acoustic liquid transfer device for obtaining at least one discrete colony from micro organisms or cells comprised in a solution. The present invention also relates to an automated method for determining the presence and/or quantity of microorganisms or cells potentially comprised in a solution and to the use of an acoustic liquid transfer device for determining the presence and/or quantity of microorganisms or cells potentially comprised in a solution.

公开(公告)号：US11248223B2

公开(公告)日：2022-02-15

申请号：US16012751

申请日：2016-12-23

申请人： CureVac AG

发明人： Benyamin Yazdan Panah ,  Aniela Wochner ,  Tilmann Roos ,  Andreas Funkner ,  Martin Kunze

IPC分类号： C12N15/10 ,  C12P19/34 ,  C12Q1/6844

摘要： The present invention relates to a buffer system comprising a dicarboxylic acid or tricarboxylic acid or a salt thereof for synthesizing RNA molecules as well as a method of RNA in vitro transcription using this buffer system. The present invention also provides the use of this buffer system in RNA in vitro transcription and in the reduction or prevention of precipitates during RNA in vitro transcription.