公开(公告)号：US20250058298A1

公开(公告)日：2025-02-20

申请号：US18712580

申请日：2022-12-06

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Åsa Hagner McWhirter ,  Ola Lind ,  Ida Helena Vestin ,  Erik Tingborn ,  Björn Norén

IPC: B01J20/28 ,  B01J20/24 ,  B01J20/282 ,  B01J20/32

Abstract: The present disclosure is directed to a separation matrix comprising a plurality of chromatography particles, each chromatography particle comprising a core and a layer surrounding the core, wherein the core has a first average pore diameter and the layer surrounding the core has a second average pore diameter, wherein the second average pore diameter is at least 1.5 times higher than the first average pore diameter, wherein the first average pore diameter excludes diffusion of a target molecule through the pores of the core and wherein the second average pore diameter at least partly permits diffusion of the target molecule through the pores of the layer surrounding the core. Further disclosed are a method for preparing such a separation matrix, uses of such a separation matrix and methods for separating target molecules by use of such a separation matrix, in particular a method for separating adeno associated virus capsids fully packaged with genetic material from adeno associated virus capsids not fully packaged with genetic material, and compositions obtained by said method.