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公开(公告)号：US20230094241A1
公开(公告)日：2023-03-30
申请号：US18056581
申请日：2022-11-17
摘要： A parallel assembly of chromatography column modules connected in a rigid housing the assembly having one common assembly inlet and one common assembly outlet each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules in the rigid housing are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.
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公开(公告)号：US11529570B2
公开(公告)日：2022-12-20
申请号：US17102801
申请日：2020-11-24
摘要： A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b) connected in a rigid housing (21; 61), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules in the rigid housing are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.
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公开(公告)号：US20220315647A1
公开(公告)日：2022-10-06
申请号：US17604964
申请日：2020-04-29
发明人： Gustav Rodrigo , Mats Ander , Tomas Bjorkman , Ronnie Palmgren , Charlotte Brink
摘要： The invention discloses a method for separation of antibodies or antibody fragments, comprising the steps of: a) providing a feed comprising antibodies or antibody fragments having a VH3 region and being devoid of an Fc region capable of binding to Protein A; b) contacting the feed with a separation resin having covalently coupled ligands, wherein the ligands comprise a polypeptide as defined by SEQ ID NO 1 and wherein the antibodies or antibody fragments bind to the separation resin; c) optionally washing the separation resin with a washing liquid; d) eluting the antibodies or antibody fragments from the separation resin with an elution liquid and recovering the antibodies or antibody fragments.
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公开(公告)号：US11439926B2
公开(公告)日：2022-09-13
申请号：US16701942
申请日：2019-12-03
发明人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Tobias E. Soderman
IPC分类号： C07K1/20 , B01D15/32 , B01J20/285 , B01J20/287 , B01J39/26 , B01J20/28 , B01J20/32 , C07K16/00 , C12N7/00 , G01N1/40
摘要： The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.
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公开(公告)号：US20220258130A1
公开(公告)日：2022-08-18
申请号：US17735882
申请日：2022-05-03
IPC分类号： B01J20/289 , B01D15/38 , B01J20/28 , B01J20/32
摘要： The present invention relates to a novel chromatography media, more closely a novel IMAC (Immobilized Metal Affinity Chromatography) media. The novel chromatography media comprises a pentaligand and provides high dynamic binding capacity as well as high purity of the sample proteins purified on the media of the invention.
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公开(公告)号：US11352388B2
公开(公告)日：2022-06-07
申请号：US15564224
申请日：2016-04-04
发明人： Martin Hall , Mikael Berg , Ida Eklind , Sandeep Kristiansson
摘要： The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of Factor VIII and von Willebrand factor from a cryoprecipitate of plasma. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.
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公开(公告)号：US11141681B2
公开(公告)日：2021-10-12
申请号：US15458667
申请日：2017-03-14
发明人： Klaus Gebauer
摘要： The invention relates to a method for providing an aseptic chromatography column, said method comprising the steps of: pre-sterilize an empty chromatography column; pre-sterilize a chromatography medium; introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thereby providing an aseptic chromatography column comprising chromatography medium.
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公开(公告)号：US20210292788A1
公开(公告)日：2021-09-23
申请号：US17047843
申请日：2019-04-24
摘要： The present invention relates to a method for virus purification. The present invention provides downstream processes for purification of adenovirus from cell culture harvest. More closely, it relates to a method for adenovirus purification using a virus capture and a virus polishing step.
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公开(公告)号：US11124539B2
公开(公告)日：2021-09-21
申请号：US16348534
申请日：2017-11-14
发明人： Johan William Ohman , Mats Ander , Therese Graner , Anneli Jorsback , Jinyu Zou
IPC分类号： C07K1/22 , B01J20/32 , B01D15/38 , B01J20/24 , B01J20/285 , B01J20/286
摘要： The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.
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公开(公告)号：US20210163529A1
公开(公告)日：2021-06-03
申请号：US16955119
申请日：2018-12-13
发明人： Gustav Rodrigo , Mats Ander , Ronnie Palmgren , Tomas Bjorkman
IPC分类号： C07K1/22 , B01J20/24 , B01J20/28 , B01J20/288 , B01J20/32 , B01J20/30 , C07K16/00 , B01D15/38
摘要： A method for preparation of a separation matrix, comprising the steps of: a) providing a solid support and an alkali-stable ligand derived from an immunoglobulin-binding bacterial protein; b) reacting said alkali-stable ligand with said solid support to form a separation matrix having covalently coupled alkali-stable ligands; and c) washing said separation matrix having covalently coupled alkali-stable ligands with a wash solution comprising at least 10 mM of an alkali metal hydroxide.
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