公开(公告)号：US20240391984A1

公开(公告)日：2024-11-28

申请号：US18682409

申请日：2022-08-24

Applicant: Cytiva Bioprocess R&D AB

Inventor： Mats Ander ,  Joakim Galli ,  Andreas L. Jonsson ,  Gustav Rodrigo

IPC: C07K16/12 ,  C07K1/22

Abstract: The present relates to a polypeptide that binds to an immunoglobulin or a fragment thereof. More specifically, it relates to a kappa light-chain binding polypeptide with high binding affinity and improved alkali stability. The one kappa light-chain binding comprises a mutated binding domain of Peptostreptococcus Protein L, derived from any one of the amino acid sequences SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17 or SEQ ID NO:18, said amino acid sequences having N6H, N41H and N56Y or N56Q mutations.