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公开(公告)号：US11136359B2
公开(公告)日：2021-10-05
申请号：US15900419
申请日：2018-02-20
Applicant: Cytiva BioProcess R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Tomas Bjorkman , Goran Bauren
IPC: C07K14/31 , B01D15/38 , B01J20/286 , B01J20/32 , C07K1/22 , C07K14/745 , C07K16/00
Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.
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公开(公告)号：US11566082B2
公开(公告)日：2023-01-31
申请号：US15525688
申请日：2015-11-11
Applicant: Cytiva BioProcess R&D AB
Inventor： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.
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公开(公告)号：US11517879B2
公开(公告)日：2022-12-06
申请号：US17107600
申请日：2020-11-30
Applicant: Cytiva Bioprocess R&D AB
Inventor： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC: B01J20/24 , B01D15/38 , B01J20/286 , C07K1/22 , B01J20/32 , C07K14/31 , C07K16/00 , C07K17/00 , B01J20/28 , B01J20/285 , B01J20/289 , C07K17/10
Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIF) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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公开(公告)号：US20240391984A1
公开(公告)日：2024-11-28
申请号：US18682409
申请日：2022-08-24
Applicant: Cytiva Bioprocess R&D AB
Inventor： Mats Ander , Joakim Galli , Andreas L. Jonsson , Gustav Rodrigo
Abstract: The present relates to a polypeptide that binds to an immunoglobulin or a fragment thereof. More specifically, it relates to a kappa light-chain binding polypeptide with high binding affinity and improved alkali stability. The one kappa light-chain binding comprises a mutated binding domain of Peptostreptococcus Protein L, derived from any one of the amino acid sequences SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17 or SEQ ID NO:18, said amino acid sequences having N6H, N41H and N56Y or N56Q mutations.
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公开(公告)号：US20220315647A1
公开(公告)日：2022-10-06
申请号：US17604964
申请日：2020-04-29
Applicant: Cytiva Bioprocess R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Tomas Bjorkman , Ronnie Palmgren , Charlotte Brink
Abstract: The invention discloses a method for separation of antibodies or antibody fragments, comprising the steps of: a) providing a feed comprising antibodies or antibody fragments having a VH3 region and being devoid of an Fc region capable of binding to Protein A; b) contacting the feed with a separation resin having covalently coupled ligands, wherein the ligands comprise a polypeptide as defined by SEQ ID NO 1 and wherein the antibodies or antibody fragments bind to the separation resin; c) optionally washing the separation resin with a washing liquid; d) eluting the antibodies or antibody fragments from the separation resin with an elution liquid and recovering the antibodies or antibody fragments.
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Patent Agency Ranking
公开(公告)号：US20210163529A1
公开(公告)日：2021-06-03
申请号：US16955119
申请日：2018-12-13
Applicant: Cytiva Bioprocess R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Ronnie Palmgren , Tomas Bjorkman
Abstract: A method for preparation of a separation matrix, comprising the steps of: a) providing a solid support and an alkali-stable ligand derived from an immunoglobulin-binding bacterial protein; b) reacting said alkali-stable ligand with said solid support to form a separation matrix having covalently coupled alkali-stable ligands; and c) washing said separation matrix having covalently coupled alkali-stable ligands with a wash solution comprising at least 10 mM of an alkali metal hydroxide.
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公开(公告)号：US20230391850A1
公开(公告)日：2023-12-07
申请号：US18249525
申请日：2021-11-10
Applicant: Cytiva BioProcess R&D AB
Inventor： Mats Ander , Gustav Rodrigo , Tomas Björkman
CPC classification number: C07K16/065 , B01D15/3809 , B01J20/286 , B01J20/3212 , B01J20/3274 , B01J20/28038 , C07K14/31
Abstract: The invention relates to an alkali-stable mutated Fc-binding Protein A domain, having at least 95% identity to SEQ ID NO: 8 or SEQ ED NO: 9.
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公开(公告)号：US11680081B2
公开(公告)日：2023-06-20
申请号：US16955119
申请日：2018-12-13
Applicant: Cytiva Bioprocess R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Ronnie Palmgren , Tomas Bjorkman
CPC classification number: C07K1/22 , B01D15/3809 , B01J20/24 , B01J20/288 , B01J20/28016 , B01J20/3071 , B01J20/3212 , B01J20/3217 , B01J20/3274 , B01J20/3295 , C07K16/00 , B01J2220/54
Abstract: Methods that include providing and reacting a solid support and an alkali-stable ligand derived from an immunoglobulin-binding bacterial protein to form a separation matrix having covalently coupled alkali-stable ligands; and washing with a wash solution comprising at least 10 mM of an alkali metal hydroxide.
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公开(公告)号：US20220362744A1
公开(公告)日：2022-11-17
申请号：US17853514
申请日：2022-06-29
Applicant: Cytiva BioProcess R&D AB
Inventor： Ronnie Palmgren , Jean-Luc Maloisel , Gustav Rodrigo , Tomas Bjorkman
Abstract: A separation matrix comprising porous particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is above 5 mg/ml, the volume-weighted median diameter of said porous particles is at least 10 and below 30 μm and the said porous particles have a gel phase distribution coefficient, expressed as KD for dextran of molecular weight 110 kDa, of 0.5-0.9.
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公开(公告)号：US20210147577A1
公开(公告)日：2021-05-20
申请号：US17149830
申请日：2021-01-15
Applicant: Cytiva BioProcess R&D AB
Inventor： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.
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