公开(公告)号：US20210011013A1

公开(公告)日：2021-01-14

申请号：US16980724

申请日：2018-05-18

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Fang CHENG ,  Jicheng DONG ,  Bingbing SUN ,  Huanan WANG

IPC: G01N33/543 ,  C12Q1/6837 ,  C07C317/18 ,  C07C315/04 ,  G01N21/64

Abstract: The present disclosure discloses a novel biochip substrate, a preparation method and an application thereof. The surface of the novel biochip substrate contains active vinyl sulfone groups. The preparation method involves a one-step reaction of a compound containing vinyl sulfone groups on both ends with a silicon-hydroxyl group on the surface of a silicon-based biochip substrate material under catalytic conditions, to prepare the biochip substrate. The application immobilizes biomacromolecules by conducting Michael addition of amino or sulfydryl group in biomacromolecules and the vinyl sulfone group on the surface of the biochip substrate, realizing biological functionalization thereof. The biochip substrate has high-density active vinyl sulfone groups, which can be used for immobilization of various biomolecules with mild fixation conditions and simple operation. The preparation method does not require complex pretreatment processes, and has high operability and reproducibility, low cost, mild reaction conditions, simple operation, and environmentally friendly, which is a broad-spectrum biochip substrate with great potential.

公开(公告)号：US20200238254A1

公开(公告)日：2020-07-30

申请号：US16486765

申请日：2018-05-18

Applicant: Dalian University of Technology

Inventor： Fang CHENG ,  Mingyang LI ,  Wei HE ,  Bingbing SUN ,  Xiaochun MA ,  Jingping QU

IPC: B01J20/26 ,  B01J20/28 ,  C07K1/22

Abstract: In a method to functionalize high-density chromatography matrix functionalization method, the hydrophilic microsphere having a surface polyhydroxy structure or the hydrophilic microsphere coated with a polyhydroxy polymer is used as chromatography matrix, under anhydrous conditions with catalyst, catalyze surface hydroxyl group of the matrix to react with divinyl sulfone, and achieve functionalization of the hydroxyl group on the surface of the matrix. The chromatography matrix surface functionalization method of the present disclosure has few steps, simple process and high functional density, and the reagents and solvents used are conventional reagents and can be recycled and reused. No byproduct is generated during the reaction with high atom economy and low cost, and the density control can be achieved by adjusting the reaction time and catalyst type.