公开(公告)号：US20210011013A1

公开(公告)日：2021-01-14

申请号：US16980724

申请日：2018-05-18

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Fang CHENG ,  Jicheng DONG ,  Bingbing SUN ,  Huanan WANG

IPC: G01N33/543 ,  C12Q1/6837 ,  C07C317/18 ,  C07C315/04 ,  G01N21/64

Abstract: The present disclosure discloses a novel biochip substrate, a preparation method and an application thereof. The surface of the novel biochip substrate contains active vinyl sulfone groups. The preparation method involves a one-step reaction of a compound containing vinyl sulfone groups on both ends with a silicon-hydroxyl group on the surface of a silicon-based biochip substrate material under catalytic conditions, to prepare the biochip substrate. The application immobilizes biomacromolecules by conducting Michael addition of amino or sulfydryl group in biomacromolecules and the vinyl sulfone group on the surface of the biochip substrate, realizing biological functionalization thereof. The biochip substrate has high-density active vinyl sulfone groups, which can be used for immobilization of various biomolecules with mild fixation conditions and simple operation. The preparation method does not require complex pretreatment processes, and has high operability and reproducibility, low cost, mild reaction conditions, simple operation, and environmentally friendly, which is a broad-spectrum biochip substrate with great potential.

公开(公告)号：US20230405591A1

公开(公告)日：2023-12-21

申请号：US18249801

申请日：2021-05-19

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Huanan WANG ,  Haoyue ZHANG ,  Chuanfeng AN

IPC: B01L3/00

CPC classification number: B01L3/502746 ,  B01L2200/10 ,  B01L2300/0883

Abstract: A multi-channel integrated microfluidic chip has at least two layers of channel structures. Each layer of channel structure is provided with a liquid phase input channel. One layer of channel structure is provided with a drop-maker unit and a collection channel. The liquid phase input channel has a liquid phase input port (1) and a resistance control unit (2). The drop-maker unit contains a multiphase emulsification channel (6) and a local resistance control unit (8). The collection channel has a washing channel (9), a washing phase input port (10) and a product output port (11). A method for preparing monodisperse gel microspheres is also provided.

公开(公告)号：US20250135426A1

公开(公告)日：2025-05-01

申请号：US18564237

申请日：2021-11-30

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Huanan WANG ,  Zhenzhen DOU ,  Kaiwen CHEN ,  Xin LI ,  Chuanbo GONG ,  Kai SUN

IPC: B01J13/00 ,  B33Y10/00 ,  B33Y50/00 ,  B33Y70/10 ,  B33Y80/00

Abstract: The present invention relates to the field of materials science, the field of nanomaterials, and the field of biomedical engineering, and in particular to an inorganic non-metallic nanoparticle-assembled hydrogel material and an application thereof inl additive manufacturing technology. The hydrogel material is assembled from inorganic non-metallic particles, so as to form a hydrogel network; the size of the inorganic non-metallic particles ranges from 10 nm to 20 um; the inorganic non-metallic particles account for 2-80 wt % of the total mass of hydrogel; and the hydrogel network has microscopic pores having a pore size ranging from 0.1 um to 30 um. The inorganic non-metallic particles are assembled into a hydrogel material by an electrostatic assembly method or a hydrophobic action assembly method or a magnetic action assembly method. The hydrogel material is additively manufactured to obtain a gel scaffold which is used as a bone repair scaffold or a cartilage repair scaffold. The hydrogel material of the present invention is directly applied to inorganic non-metallic additive manufacturing technology, without using an additive or a cross-linking agent.

公开(公告)号：US20240100224A1

公开(公告)日：2024-03-28

申请号：US18263046

申请日：2021-05-19

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Huanan WANG ,  Kaiwen CHEN

IPC: A61L27/52 ,  A61L24/00 ,  A61L24/10 ,  A61L27/22 ,  A61L27/56 ,  A61L31/04 ,  A61L31/14

CPC classification number: A61L27/52 ,  A61L24/0031 ,  A61L24/0036 ,  A61L24/104 ,  A61L27/222 ,  A61L27/56 ,  A61L31/045 ,  A61L31/145 ,  A61L31/146 ,  A61L2300/424 ,  A61L2400/04 ,  A61L2400/06 ,  A61L2430/02

Abstract: An injectable, moldable and printable gramular hydrogel material crosslinked by non-covalent and covalent bonds, a preparation method therefor, and applications thereof are provided. The material uses gelatin particles or particles that have core-shell structures as basic structural units, and forms a continuous and porous particle network by means of the reversible non-covalent crosslinking and covalent bond crosslinking of the particles. The gelatin particles or the particles that have core-shell structures form a continuous porous particle network by means of reversible self-assembly under the effect of the non-covalent bonds, thus achieving injectable, printable, moldable and self-healing properties. Furthermore, high strength granular hydrogels are formed by means of initiating covalent crosslinking. The material can be used as a drug-sustained release carrier, a tissue engineering scaffold and a tissue adhesive hemostatic material in the field of biomedicine.