公开(公告)号：US09862731B2

公开(公告)日：2018-01-09

申请号：US15039674

申请日：2014-11-26

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Jiangli Fan ,  Hao Zhu ,  Xiaojun Peng ,  Jingyun Wang

IPC: C07F5/02 ,  C09K11/06 ,  G01N33/58 ,  G01N33/84

CPC classification number: C07F5/022 ,  C07F5/02 ,  C09K11/06 ,  C09K2211/1022 ,  C09K2211/1029 ,  G01N33/582 ,  G01N33/84

Abstract: The present invention provides a difluoroboron dipyrromethene fluorescent probe having a structure represented by the following general formula I, and a production method and an application thereof. Said difluoroboron dipyrromethene fluorescent probe exhibits low background fluorescence, and a rapid and significant fluorescence enhancement up to 100 times after the addition of hypochloric acid. The fluorescent intensity of said fluorescent probe shows a good linear relation with the concentration of hypochloric acid in a nanomole level, and the detection limit thereof is 0.56 nanomole. Said fluorescent probe has good selectivity since it hardly responds to other reactive oxygen species such as H2O2, O2−, TBHP, HO., TBO., 1O2 and NO., and is not interfered by pH in a wide range. Said fluorescent probe can be applied to detect hypochloric acid in living cells.