公开(公告)号：US20220002685A1

公开(公告)日：2022-01-06

申请号：US17024340

申请日：2020-09-17

Applicant: DANISCO US INC

Inventor： Slavko KRALJ ,  Marc Kolkman ,  Chris Leeflang ,  Johannes G. De Nobel ,  Arjen Hoekstra ,  Veli Alkan

IPC: C12N9/10 ,  C12P19/18 ,  A61K35/742 ,  A61K38/45

Abstract: Bacillus agaradhaerens strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.

公开(公告)号：US11859216B2

公开(公告)日：2024-01-02

申请号：US17024340

申请日：2020-09-17

Applicant: DANISCO US INC

Inventor： Slavko Kralj ,  Marc Kolkman ,  Chris Leeflang ,  Johannes G. De Nobel ,  Arjen Hoekstra ,  Veli Alkan

IPC: C12N9/10 ,  A61K35/74 ,  C12P19/18 ,  A61K35/742 ,  A61K38/45

CPC classification number: C12N9/1051 ,  A61K35/742 ,  A61K38/45 ,  C12N9/1048 ,  C12P19/18 ,  C12Y204/01009

Abstract: Bacillus agaradhaerens strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.