公开(公告)号：US20190264247A1

公开(公告)日：2019-08-29

申请号：US16281225

申请日：2019-02-21

Applicant: DANISCO US INC

Inventor： Zheyong Yu ,  Slavko Kralj ,  Zhenghong Zhang ,  Laurie A. Howe

IPC: C12P19/08 ,  C12N9/10

Abstract: Reaction compositions are disclosed herein comprising at least water, beta-glucose-1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.

公开(公告)号：US09523087B2

公开(公告)日：2016-12-20

申请号：US14189943

申请日：2014-02-25

Applicant: Danisco US Inc.

Inventor： Wolfgang Aehle ,  Richard R. Bott ,  Benjamin S. Bower ,  Jonathan Caspi ,  David A. Estell ,  Frits Goedegebuur ,  Ronaldus Wilhelmus Joannes Hommes ,  Thijs Kaper ,  Bradley R. Kelemen ,  Slavko Kralj ,  Johannes Franciscus Thomas Van Lieshout ,  Igor Nikolaev ,  Sander Van Stigt Thans ,  Louise Wallace ,  Gudrun Vogtentanz ,  Mats Sandgren

IPC: C12N9/42 ,  C12N9/52 ,  C07H21/04

CPC classification number: C12N9/2437 ,  C12Y302/01091 ,  Y02E50/17

Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.

Abstract translation: 本公开涉及纤维素酶变体。 特别地，本公开涉及具有改善的表达，活性和/或稳定性的纤维素酶变体。 还描述了编码纤维素酶变体的核酸，包含纤维素酶变体的组合物及其使用方法。

公开(公告)号：US11180740B2

公开(公告)日：2021-11-23

申请号：US16383820

申请日：2019-04-15

Applicant: DANISCO US INC

Inventor： Zheyong Yu ,  Slavko Kralj ,  Natnael Behabtu ,  Zhenghong Zhang ,  Laurie A Howe

IPC: C12N9/10 ,  C12P19/04 ,  C08B37/00

Abstract: Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.

公开(公告)号：US10808269B2

公开(公告)日：2020-10-20

申请号：US16281225

申请日：2019-02-21

Applicant: DANISCO US INC

Inventor： Zheyong Yu ,  Slavko Kralj ,  Zhenghong Zhang ,  Laurie A. Howe

IPC: C12P19/08 ,  C12N9/10 ,  C12P19/04 ,  C12P19/18

Abstract: Reaction compositions are disclosed herein comprising at least water, beta-glucose-1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.

公开(公告)号：US09938551B2

公开(公告)日：2018-04-10

申请号：US14650285

申请日：2013-12-10

Applicant: Danisco US Inc.

Inventor： Richard R. Bott ,  Maria Foukaraki ,  Ronaldus Wilhelmus Hommes ,  Thijs Kaper ,  Bradley R. Kelemen ,  Slavko Kralj ,  Igor Nikolaev ,  Mats Sandgren ,  Johannes Franciscus Thomas Van Lieshout ,  Sander Van Stigt Thans

IPC: C12P19/14 ,  C12P19/02 ,  C12N9/42

CPC classification number: C12P19/14 ,  C12N9/2434 ,  C12N9/2437 ,  C12P19/02 ,  C12Y302/01091

Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Ce17A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.

公开(公告)号：US11859216B2

公开(公告)日：2024-01-02

申请号：US17024340

申请日：2020-09-17

Applicant: DANISCO US INC

Inventor： Slavko Kralj ,  Marc Kolkman ,  Chris Leeflang ,  Johannes G. De Nobel ,  Arjen Hoekstra ,  Veli Alkan

IPC: C12N9/10 ,  A61K35/74 ,  C12P19/18 ,  A61K35/742 ,  A61K38/45

CPC classification number: C12N9/1051 ,  A61K35/742 ,  A61K38/45 ,  C12N9/1048 ,  C12P19/18 ,  C12Y204/01009

Abstract: Bacillus agaradhaerens strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.

公开(公告)号：US11802275B2

公开(公告)日：2023-10-31

申请号：US17261937

申请日：2019-07-18

Applicant: DANISCO US INC

Inventor： Slavko Kralj ,  Zheyong Yu ,  Zhenghong Zhang

IPC: C12P19/04 ,  C12N9/10

CPC classification number: C12N9/1051 ,  C12P19/04 ,  C12Y204/01001 ,  C12Y204/01007

Abstract: Reactions are disclosed herein comprising water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and an alpha-1,4-glucan phosphorylase. Novel alpha-1,4-glucan phosphorylase enzymes are also disclosed. Additional disclosures herein regard sucrose phosphorylase enzymes and methods of use thereof to produce alpha-G1P.

公开(公告)号：US10081802B2

公开(公告)日：2018-09-25

申请号：US14909007

申请日：2014-07-24

Applicant: DANISCO US INC.

Inventor： Maria Foukaraki ,  Ronaldus Wilhelmus Hommes ,  Thijs Kaper ,  Bradley R. Kelemen ,  Slavko Kralj ,  Suzanne E. Lantz ,  Igor Nikolaev ,  Wilhelmus Van Der Kley ,  Johannes Franciscus Thomas Van Lieshout ,  Sander Van Stigt Thans

IPC: C12N9/42 ,  C12P19/14 ,  C12P19/02 ,  C12N9/24

Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.

公开(公告)号：US20170362621A1

公开(公告)日：2017-12-21

申请号：US15536915

申请日：2015-12-18

Applicant: Danisco US Inc.

Inventor： Zachary Q. Beck ,  Meredith K. Fujdala ,  Henrik Hansson ,  Thijs Kaper ,  Slavko Kralj ,  Amy D. Liu ,  Nils Egil Mikkelsen ,  Mats Sandgren

IPC: C12P19/14 ,  C12N9/24 ,  C12P19/02 ,  C12N9/42

CPC classification number: C12P19/14 ,  C12N9/2445 ,  C12N9/248 ,  C12P19/02 ,  C12Y302/01021 ,  C12Y302/01037

Abstract: Provided are certain glycosyl hydrolase family 3 (GH3) beta-glucosidase enzymes engineered to acquire beta-xylosidase activities. Provided also are compositions comprising multi-functional GH3 enzymes and methods of use or industrial applications thereof.

公开(公告)号：US20220195399A1

公开(公告)日：2022-06-23

申请号：US17528564

申请日：2021-11-17

Applicant: DANISCO US INC

Inventor： Zheyong Yu ,  Slavko Kralj ,  Natnael Behabtu ,  Zhenghong Zhang ,  Laurie A. Howe

IPC: C12N9/10 ,  C08B37/00 ,  C12P19/04

Abstract: Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.