公开(公告)号：US20160168582A1

公开(公告)日：2016-06-16

申请号：US14857343

申请日：2015-09-17

申请人： DANISCO US INC.

发明人： Eugenio FERRARI ,  Carole HARBISON ,  M. Harunur RASHID ,  Walter WEYLER

IPC分类号： C12N15/75 ,  C12N9/54

CPC分类号： C12N15/75 ,  C07K14/32 ,  C12N9/54 ,  C12P21/02 ,  C12R1/125 ,  C12Y304/00

摘要： The present invention provides cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms, such as Bacillus species having enhanced expression of a protein of interest, wherein one or more chromosomal genes have been inactivated, and preferably wherein one or more chromosomal genes have been deleted from the Bacillus chromosome. In some further embodiments, one or more indigenous chromosomal regions have been deleted from a corresponding wild-type Bacillus host chromosome.

摘要翻译： 本发明提供已被遗传操作以具有改变产生表达蛋白质的能力的细胞。 特别地，本发明涉及革兰氏阳性微生物，例如具有增强的感兴趣蛋白表达的芽孢杆菌属物种，其中一个或多个染色体基因已被灭活，并且优选其中一个或多个染色体基因已经从芽孢杆菌中缺失 染色体。 在一些另外的实施方案中，一个或多个本征染色体区已经从相应的野生型芽孢杆菌宿主染色体中缺失。