公开(公告)号：US3687927A

公开(公告)日：1972-08-29

申请号：US3687927D

申请日：1971-06-07

Applicant: DIAGNOSTIC DATA INC

Inventor： HUBER WOLFGANG

IPC: A61K38/00 ,  C12N9/02 ,  C07G7/04

CPC classification number: C12N9/0089 ,  A61K38/00 ,  Y10S530/829 ,  Y10S530/846

Abstract: An improved process for reducing the divalent metal ion content to less than 10 7 M concentration of a solution of orgotein containing greater than 10 7 M concentration of a divalent metal having an ionic radius from 0.65 to 0.79 A, which comprises reducing the ion content to less than 10 7 M by passing the buffer solution having an ion content greater than 10 7 M through an ion exchange resin bed consisting of a single ion exchange resin having both acidic and basic groups which are in sufficiently close proximity so that the resin is partially neutralized internally yet sufficiently separated spatially so that the resin has absorptive attraction for both anions and cations.

Abstract translation: 一种用于将二价金属离子含量降低至低于10-7M浓度的含有大于10-7M浓度的具有0.65至0.79A的离子半径的二价金属的有机蛋白质溶液的改进方法，其中 通过使离子含量大于10 -7 M的缓冲溶液通过由具有酸性和碱性基团的单一离子交换树脂组成的离子交换树脂床将离子含量降低至小于10-7M 它们足够靠近，使得树脂在内部部分中和在空间上充分分离，使得树脂对阴离子和阳离子都具有吸收吸引力。

公开(公告)号：US3579495A

公开(公告)日：1971-05-18

申请号：US3579495D

申请日：1970-04-24

Applicant: DIAGNOSTIC DATA INC

Inventor： HUBER WOLFGANG

IPC: A61K38/00 ,  C12N9/02 ,  A61K17/00 ,  C07G7/04

CPC classification number: C12N9/0089 ,  A61K38/00 ,  Y10S530/829

Abstract: ORGOTEIN IS ISOLATED FROM LYSED PLASMA-FREE ERYTHROCYTES BY A SERIES OF STEPS WHICH INCLUDE REMOVING THE HEMOGLOBIN FROM THE LYSATE, HEATING THE REMANING SOLUBLE PROTEINS IN THE PRESENCE OF BUFFER AND DIVALENT METAL ION UNTIL THE CARBONIC ANHYDRASE IS INACTIVATED, REMOVING THE PRECIPITATE, AND SEPARATING THE ORGOTEIN PROTEIN FROM THE SUPERNATANT LIQUID.

公开(公告)号：US3637640A

公开(公告)日：1972-01-25

申请号：US3637640D

申请日：1970-05-04

Applicant: DIAGNOSTIC DATA INC

Inventor： HUBER WOLFGANG

IPC: A61K9/00 ,  A61K38/00 ,  A61K47/26 ,  C12N9/02 ,  C07G7/04

CPC classification number: C12N9/0089 ,  A61K9/0019 ,  A61K38/00 ,  A61K47/26 ,  Y10S206/828 ,  Y10S530/846

Abstract: SUCROSE, GLUCOSE, MANNOSE, AND CERTAIN PENTOSE HEXOSE AND HEPTOSE SACCHARIDES IMPROVE THE SABILITY OF ORGOTEIN TO LYOPHILIZATION AND STORAGE. ORGOTEIN IS THE NAME ASSIGNED BY USCAN TO AN ISOLATED, SUBSTANITIALLY PURE, SOLUBLE, GLOBULAR, NON-TOXIC AND SUBSTANTIALLY NON-ANTIGENIC METALLOPROTEIN HAVING ANTI-INFLAMMATORY AND OTHER PHARMACODYNAMIC ACTIVITY.

公开(公告)号：US3637641A

公开(公告)日：1972-01-25

申请号：US3637641D

申请日：1970-01-16

Applicant: DIAGNOSTIC DATA INC

Inventor： HUBER WOLFGANG ,  SCHULTE THOMAS L

IPC: A61K38/00 ,  A61K38/44 ,  C12N9/02 ,  A61K27/00

CPC classification number: C12N9/0089 ,  A61K38/446

Abstract: THE PAIN AND STRUCTURAL ABNORMALITIES ASSOCIATED WITH POST-TRAUMATIC ARTHRITIS ARE ALLEVIATED BY SYSTEMIC ADMINISTRATION OF ORGOTEIN TO THE PATIENT AFFECTED WITH THE ARTHRITIC CONDITION.

公开(公告)号：US3624251A

公开(公告)日：1971-11-30

申请号：US3624251D

申请日：1970-01-16

Applicant: DIAGNOSTIC DATA INC

Inventor： HUBER WOLFGANG

IPC: B01J47/00 ,  C12N9/02 ,  A61K17/00 ,  C07G7/04

CPC classification number: C12N9/0089 ,  B01J47/014 ,  C12Y115/01001 ,  Y10S530/846

Abstract: Heat labile tenacious proteins present in samples of substantially pure orgotein produced by a process which comprises a heating step in which a mixture of proteins comprising the orgotein protein and substantial amounts of other proteins are heated under conditions which do not affect the orgotein, are eliminated therefrom by a second heating step of a solution of the substantially pure orgotein in a buffer containing divalent metal ions at a temperature higher than employed in the prior heating step.