公开(公告)号：US20240287116A1

公开(公告)日：2024-08-29

申请号：US18570021

申请日：2022-06-14

Applicant: DSM IP ASSETS B.V.

Inventor： Nikolay KHANZHIN ,  Pierre CHASSAGNE

IPC: C07H3/06 ,  B01J47/014 ,  C07H1/08

CPC classification number: C07H3/06 ,  B01J47/014 ,  C07H1/08

Abstract: The invention relates to a method for recovery and purification of a neutral or sialylated human milk oligosaccharide (HMO) from a fermentation broth, comprising the steps of separating the fermentation broth to form a separated HMO-containing stream and a biomass waste stream, purifying the HMO-containing stream by nanofiltration, purifying the HMO-containing stream with an acidic cation exchange resin, concentrating the purified HMO-containing stream, and drying the purified HMO-containing stream to obtain a solidified neutral or sialylated HMO. Moreover, the invention also concerns a neutral or sialylated human milk oligosaccharide obtained by the inventive method, as well as its use in food, feed, and medical application.

公开(公告)号：US20180207631A1

公开(公告)日：2018-07-26

申请号：US15847854

申请日：2017-12-19

Applicant: INVISTA North America S.a.r.l.

Inventor： Mariusz Kamionka ,  Alex Conradie

IPC: B01J49/05 ,  B01J39/18 ,  B01J47/014 ,  B01J47/11 ,  C02F1/42 ,  C07C209/86

CPC classification number: B01J49/05 ,  B01J39/05 ,  B01J39/18 ,  B01J47/014 ,  B01J47/016 ,  B01J47/11 ,  B01J49/60 ,  C02F1/20 ,  C02F1/42 ,  C02F2101/34 ,  C02F2101/38 ,  C02F2303/16 ,  C07C209/86

Abstract: Described herein are processes and apparatus for the high purity and high concentration recovery of multivalent products via continuous ion exchange from aqueous solutions for further down-stream purification.

公开(公告)号：US20170145050A1

公开(公告)日：2017-05-25

申请号：US15327161

申请日：2015-07-23

Applicant: ASAHI KASEI MEDICAL CO., LTD.

Inventor： Hiroki TANIGUCHI ,  Ichiro KOGUMA

IPC: C07K1/18 ,  B01D15/38 ,  C08J5/22 ,  B01D15/36 ,  C07K16/00 ,  C07K1/22

CPC classification number: C07K1/18 ,  B01D15/362 ,  B01D15/3809 ,  B01J39/19 ,  B01J39/26 ,  B01J47/014 ,  B01J47/12 ,  C07K1/22 ,  C07K16/00 ,  C07K2317/21 ,  C08J5/2243 ,  C08J7/18 ,  C08J9/36 ,  C08J2323/06 ,  G01N30/02 ,  G01N30/88 ,  G01N30/96

Abstract: The present invention provides a cation-exchange chromatographic support, comprising a membrane matrix and a copolymer immobilized on the surface of the membrane matrix, wherein the copolymer comprises a (meth)acrylamide-based compound and/or a (meth)acrylate-based compound as monomer units, and the support has one or more species of cation-exchange groups including at least a weak cation-exchange group at a density higher than 30 mmol/L per volume of the support.

公开(公告)号：US09029517B2

公开(公告)日：2015-05-12

申请号：US13191992

申请日：2011-07-27

Applicant: David Yavorsky ,  John Amara ,  Joaquin Umana ,  William Cataldo ,  Mikhail Kozlov ,  Matthew Stone

Inventor： David Yavorsky ,  John Amara ,  Joaquin Umana ,  William Cataldo ,  Mikhail Kozlov ,  Matthew Stone

IPC: B01J20/28 ,  B01J47/12 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/285 ,  B01J20/287 ,  B01J20/32 ,  B01J39/26 ,  B01J41/20 ,  B01J47/00

CPC classification number: B01J39/26 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28023 ,  B01J20/28059 ,  B01J20/285 ,  B01J20/287 ,  B01J20/3244 ,  B01J41/20 ,  B01J47/014 ,  B01J47/127 ,  C07K1/18 ,  C07K16/00 ,  C12N7/00 ,  C12N7/02 ,  G01N2030/521 ,  Y10S502/52724 ,  Y10T428/2938 ,  Y10T428/2973

Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

Abstract translation: 来自成形纤维的用于色谱的吸附介质，特别是离子交换层析。 在某些实施方案中，与普通纤维相比，官能化成形纤维呈现原纤化或脊状结构，其大大增加了纤维的表面积。 本文还公开了向高表面积纤维添加提供阳离子交换或阴离子交换官能团的表面侧基官能团的方法。 这种侧链官能团可用于生物分子的离子交换色谱纯化，如单克隆抗体（mAb）。

公开(公告)号：US20070112178A1

公开(公告)日：2007-05-17

申请号：US10589718

申请日：2005-02-24

Applicant: Bo-Lennart Johansson ,  Hans Johansson ,  Anders Ljunglof ,  Jean-Luc Maloisel ,  Nicolas Thevenin

Inventor： Bo-Lennart Johansson ,  Hans Johansson ,  Anders Ljunglof ,  Jean-Luc Maloisel ,  Nicolas Thevenin

IPC: C07K16/00 ,  C02F1/28

CPC classification number: C07K1/18 ,  B01D15/362 ,  B01D15/3809 ,  B01D15/3847 ,  B01J39/07 ,  B01J39/26 ,  B01J47/014 ,  C07K1/165 ,  B01D15/327

Abstract: The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.

Abstract translation: 本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个 阳离子交换基团和至少一个芳族或杂芳族环系统。 在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。 本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。

公开(公告)号：US20060154996A1

公开(公告)日：2006-07-13

申请号：US11301053

申请日：2005-12-12

Applicant: Hiroshi Inoue ,  Koji Yamanaka ,  Makio Tamura ,  Shusaku Yoshida ,  Hideo Nakamura

Inventor： Hiroshi Inoue ,  Koji Yamanaka ,  Makio Tamura ,  Shusaku Yoshida ,  Hideo Nakamura

IPC: C08J5/20

CPC classification number: B01J47/08 ,  B01D61/48 ,  B01J20/28033 ,  B01J20/28054 ,  B01J20/28069 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/28092 ,  B01J39/26 ,  B01J41/20 ,  B01J47/014 ,  B01J2220/52 ,  C02F1/42 ,  C02F1/469

Abstract: A porous ion exchanger includes an open cell structure including interconnected macropores and mesopores whose average diameter is in a range of 1 to 1000 μm existing on walls of the macropores. Moreover, a total pore volume is in a range of 1 to 50 ml/g, ion exchange groups are uniformly distributed, and an ion exchange capacity is not less than 0.5 mg equivalent/g of dry porous ion exchanger. The porous ion exchanger can be used as an ion exchanger filled into a deionization module of an electrodeionization water purification device, solid acid catalyst, adsorbent, and filler for chromatography.

公开(公告)号：US20030189005A1

公开(公告)日：2003-10-09

申请号：US10311071

申请日：2002-12-13

Inventor： Hiroshi Inoue ,  Koji Yamanaka ,  Makio Tamura ,  Shusaku Yoshida ,  Hideo Nakamura

IPC: B01D015/00

CPC classification number: B01J47/08 ,  B01D61/48 ,  B01J20/28033 ,  B01J20/28054 ,  B01J20/28069 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/28092 ,  B01J39/26 ,  B01J41/20 ,  B01J47/014 ,  B01J2220/52 ,  C02F1/42 ,  C02F1/469

Abstract: A porous ion exchanger includes an open cell structure including interconnected macropores and mesopores whose average diameter is in a range of 1 to 1000 nullm existing on walls of the macropores. Moreover, a total pore volume is in a range of 1 to 50 ml/g, ion exchange groups are uniformly distributed, and an ion exchange capacity is not less than 0.5 mg equivalent/g of dry porous ion exchanger. The porous ion exchanger can be used as an ion exchanger filled into a deionization module of an electrodeionization water purification device, solid acid catalyst, adsorbent, and filler for chromatography.

Abstract translation: 多孔离子交换器包括开孔结构，其包含互连的大孔和介孔，其平均直径在大孔壁上存在1〜1000μm的范围。 此外，总孔体积在1〜50ml / g的范围内，离子交换基团均匀分布，离子交换容量不小于0.5mg当量/ g干式多孔离子交换剂。 多孔离子交换剂可用作填充在电去离子水净化装置，固体酸催化剂，吸附剂和色谱填料的去离子模块中的离子交换剂。

公开(公告)号：US3759826A

公开(公告)日：1973-09-18

申请号：US3759826D

申请日：1971-08-23

Applicant: GEORGIA PACIFIC CORP

Inventor： FELICETTA V ,  KING E

IPC: A23C9/148 ,  B01J47/00 ,  B01D15/08

CPC classification number: A23C9/148 ,  B01J47/014 ,  Y10S530/832 ,  Y10S530/833 ,  Y10S530/859 ,  Y10T137/0391

Abstract: A PROCESS FOR THE FRACTIONATION AND RECOVERY OF WATERSOLUBLE COMPOUNDS BY GEL PERMEATION USING A WATER-INSOLUBLE, POLYMERIZED LIGNOSULFONATE RESIN.

公开(公告)号：US3340156A

公开(公告)日：1967-09-05

申请号：US44932865

申请日：1965-04-19

Applicant: NOVO TERAPEUTISK LABOR AS

Inventor： JOHANNES JENSEN VILLY

IPC: B01J47/00 ,  C12N9/68

CPC classification number: C12N9/6435 ,  B01J47/014 ,  C12Y304/21007 ,  Y10S435/816

公开(公告)号：US3337529A

公开(公告)日：1967-08-22

申请号：US45677865

申请日：1965-05-18

Applicant: SCHWARZ BIORES INC

Inventor： LOUIS LAUFER

IPC: B01J47/00 ,  C07H1/06

CPC classification number: C07H1/06 ,  B01J47/014