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公开(公告)号：US20210147867A1
公开(公告)日：2021-05-20
申请号：US17143824
申请日：2021-01-07
Applicant: DOW AGROSCIENCES LLC
Inventor： Terry WRIGHT , Justin LIRA , Donald MERLO , Nicole ARNOLD
Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
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Patent Agency Ranking
公开(公告)号：US20210254090A1
公开(公告)日：2021-08-19
申请号：US17175966
申请日：2021-02-15
Applicant: DOW AGROSCIENCES LLC
Inventor： Terry WRIGHT , Justin LIRA , Donald MERLO , Nicole ARNOLD
Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
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公开(公告)号：US20220098610A1
公开(公告)日：2022-03-31
申请号：US17403476
申请日：2021-08-16
Applicant: DOW AGROSCIENCES LLC
Inventor： Terry WRIGHT , Justin LIRA , Donald MERLO , Nicole ARNOLD
Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
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公开(公告)号：US20170211087A1
公开(公告)日：2017-07-27
申请号：US15468494
申请日：2017-03-24
Applicant: DOW AGROSCIENCES LLC
Inventor： Terry R. WRIGHT , Justin M. LIRA , Terence Anthony WALSH , Donald MERLO , Jayakumar Pon SAMUEL , Gaofeng LIN
Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
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公开(公告)号：US20170022515A1
公开(公告)日：2017-01-26
申请号：US15288406
申请日：2016-10-07
Applicant: Dow AgroSciences LLC
Inventor： Terry WRIGHT , Justin LIRA , Donald MERLO , Nicole ARNOLD
CPC classification number: C12N15/8274 , C12N9/0069 , C12N15/8209
Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
Abstract translation: 本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。 迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。 本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和 改善除草剂抗性管理方案。 更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。 据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。 这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。 本发明还包括控制杂草的相关方法。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。
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6.发明申请公开(公告)号：US20160068851A1
公开(公告)日：2016-03-10
申请号：US14844731
申请日：2015-09-03
Applicant: Dow AgroSciences LLC
Inventor： Manju GUPTA , Sara BENNETT , Sandeep KUMAR , Donald MERLO , Nagesh SARDESAI
CPC classification number: C12N15/8205 , C12N15/743
Abstract: The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.
Abstract translation: 本公开提供了用于生产和使用相对于亲本菌株缺乏RecA活性的根癌土壤杆菌菌株（例如LBA4404）的新型组合物和方法。 还公开了与根癌土壤杆菌的其他基因缺陷型菌株的组合。 具体地，提供了两个示例性的recA减毒株，UIA777，其中氯霉素抗性基因破坏recA基因和UIA770，其中卡那霉素抗性基因破坏recA基因。
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