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公开(公告)号：US20150184175A1
公开(公告)日：2015-07-02
申请号：US14587735
申请日：2014-12-31
Applicant: DOW AGROSCIENCES LLC
Inventor： Sandeep KUMAR , Manju GUPTA , Terry R. WRIGHT , Susan M. JAYNE , Doug A. SMITH , Diaa ALABED
CPC classification number: C12N15/8216 , C12N15/10
Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. B104.
Abstract translation: 玉米罐头 B73泛素-1（Z. mays c.v.B73 Ubi-1）启动子驱动植物中高水平的组成型转基因表达。 重复使用相同的Z. mays c.v. 多基因构建体中的B73 Ubi-1启动子也可导致基因沉默，从而使得转基因产品的效力较差。 提供了使用来自不同Z. mays基因型Z. mays c.v的Ubi-1启动子的基因调控元件在基因调控启动子元件，构建体和用于在植物细胞和/或植物组织中表达转基因的方法。 B104。
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2.发明申请ZEA MAYS METALLOTHIONEIN-LIKE REGULATORY ELEMENTS AND USES THEREOF 有权ZEA MAYS METALLOTHIONEIN-LIKE REGOREORY元素及其用途公开(公告)号：US20150101083A1
公开(公告)日：2015-04-09
申请号：US14505055
申请日：2014-10-02
Applicant: Dow AgroSciences LLC
Inventor： Manju GUPTA , Sandeep KUMAR , Navin ELANGO , Karthik Narayna MUTHURAMAN , Jeffrey BERINGER , Huixia WU , Nagesh SARDESAI
IPC: C12N15/82
CPC classification number: C12N15/8227 , C12N15/8216 , C12N15/8222
Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays metallothionein-like gene regulatory elements.
Abstract translation: 提供的是使用玉米金属硫蛋白样基因调控元件在植物细胞和/或植物组织中表达转基因的构建体和方法。
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公开(公告)号：US20150184180A1
公开(公告)日：2015-07-02
申请号：US14587743
申请日：2014-12-31
Applicant: DOW AGROSCIENCES LLC
Inventor： Sandeep KUMAR , Manju GUPTA , Terry R. WRIGHT , Susan M. JAYNE , Doug A. SMITH , Diaa ALABED
IPC: C12N15/82
CPC classification number: C12N15/8216
Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. LLN37.
Abstract translation: 玉米罐头 B73泛素-1（Z. mays c.v.B73 Ubi-1）启动子驱动植物中高水平的组成型转基因表达。 重复使用相同的Z. mays c.v. 多基因构建体中的B73 Ubi-1启动子也可导致基因沉默，从而使得转基因产品的效力较差。 提供了使用来自不同Z. mays基因型Z. mays c.v的Ubi-1启动子的基因调控元件在基因调控启动子元件，构建体和用于在植物细胞和/或植物组织中表达转基因的方法。 LLN37。
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4.发明申请CONSTRUCTS FOR EXPRESSING TRANSGENES USING REGULATORY ELEMENTS FROM SETARIA UBIQUITIN GENES 有权使用来自SETARIA UBIQUITIN基因的调节元素表达转基因的构建公开(公告)号：US20150067926A1
公开(公告)日：2015-03-05
申请号：US14472969
申请日：2014-08-29
Applicant: Dow AgroSciences, LLC
Inventor： Sandeep KUMAR , Andrew ASBERRY
IPC: C12N15/82
CPC classification number: C12N15/8216 , C07K14/415
Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Setaria italica ubiquitin genes.
Abstract translation: 提供了使用调节元件（包括分离自Setaria italica泛素基因的启动子和/或3'-UTR）在植物细胞和/或植物组织中表达转基因的构建体和方法。
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5.发明申请CONSTRUCTS FOR EXPRESSING TRANSGENES USING REGULATORY ELEMENTS FROM PANICUM UBIQUITIN GENES 有权使用来自泛素基因的调节元素表达转基因的构建公开(公告)号：US20150067924A1
公开(公告)日：2015-03-05
申请号：US14472957
申请日：2014-08-29
Applicant: Dow AgroSciences, LLC
Inventor： Sandeep KUMAR , Andrew F. WORDEN
IPC: C12N15/82
CPC classification number: C12N15/8216 , C07K14/415
Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Panicum virgatum ubiquitin genes.
Abstract translation: 提供了使用调节元件（包括从黍属泛素泛素基因分离的启动子和/或3'-UTR）在植物细胞和/或植物组织中表达转基因的构建体和方法。
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Patent Agency Ranking
公开(公告)号：US20150184176A1
公开(公告)日：2015-07-02
申请号：US14587762
申请日：2014-12-31
Applicant: DOW AGROSCIENCES LLC
Inventor： Sandeep KUMAR , Manju GUPTA , Terry R. WRIGHT , Susan M. JAYNE , Doug A. SMITH , Diaa ALABED
CPC classification number: C12N15/8216 , C12N15/10
Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v2.
Abstract translation: 玉米罐头 B73泛素-1（Z. mays c.v.B73 Ubi-1）启动子驱动植物中高水平的组成型转基因表达。 重复使用相同的Z. mays c.v. 多基因构建体中的B73 Ubi-1启动子也可导致基因沉默，从而使得转基因产品的效力较差。 本发明提供了使用来自不同玉蜀黍属Z. luxurians v2的Ubi-1启动子的基因调控元件在植物细胞和/或植物组织中表达转基因的基因调控启动子元件，构建体和方法。
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7.发明申请CONSTRUCTS FOR EXPRESSING TRANSGENES USING REGULATORY ELEMENTS FROM BRACHYPODIUM UBIQUITIN 1C GENES 有权使用来自BRACHYPODIUM UBIQUITIN 1C基因的调节元件表达转基因的构建体公开(公告)号：US20150067927A1
公开(公告)日：2015-03-05
申请号：US14472977
申请日：2014-08-29
Applicant: Dow AgroSciences, LLC
Inventor： Sandeep KUMAR , Manju GUPTA , Diaa ALABED
IPC: C12N15/82
CPC classification number: C12N15/8216 , C07K14/415
Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Brachypodium distachyon ubiquitin genes.
Abstract translation: 提供了使用调节元件（包括分离自Twachypodium distachyon泛素基因的启动子和/或3'-UTR）在植物细胞和/或植物组织中表达转基因的构建体和方法。
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8.发明申请CONSTRUCTS FOR EXPRESSING TRANSGENES USING REGULATORY ELEMENTS FROM BRACHYPODIUM UBIQUITIN GENES 有权使用来自BRACHYPODIUM UBIQUITIN基因的调节元件表达转基因的构建公开(公告)号：US20150067925A1
公开(公告)日：2015-03-05
申请号：US14472965
申请日：2014-08-29
Applicant: Dow AgroSciences, LLC
Inventor： Sandeep KUMAR , Jeffrey BERINGER
IPC: C12N15/82
CPC classification number: C12N15/8216 , C07K14/415
Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Brachypodium distachyon ubiquitin genes.
Abstract translation: 提供了使用调节元件（包括分离自Twachypodium distachyon泛素基因的启动子和/或3'-UTR）在植物细胞和/或植物组织中表达转基因的构建体和方法。
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公开(公告)号：US20180171345A1
公开(公告)日：2018-06-21
申请号：US15887723
申请日：2018-02-02
Applicant: Dow AgroSciences LLC
Inventor： Sandeep KUMAR , Manju GUPTA , Terry R. WRIGHT , Susan M. JAYNE , Doug A. SMITH , Diaa ALABED
CPC classification number: C12N15/8216 , C12N15/10
Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v2.
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10.发明申请公开(公告)号：US20160068851A1
公开(公告)日：2016-03-10
申请号：US14844731
申请日：2015-09-03
Applicant: Dow AgroSciences LLC
Inventor： Manju GUPTA , Sara BENNETT , Sandeep KUMAR , Donald MERLO , Nagesh SARDESAI
CPC classification number: C12N15/8205 , C12N15/743
Abstract: The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.
Abstract translation: 本公开提供了用于生产和使用相对于亲本菌株缺乏RecA活性的根癌土壤杆菌菌株（例如LBA4404）的新型组合物和方法。 还公开了与根癌土壤杆菌的其他基因缺陷型菌株的组合。 具体地，提供了两个示例性的recA减毒株，UIA777，其中氯霉素抗性基因破坏recA基因和UIA770，其中卡那霉素抗性基因破坏recA基因。
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