公开(公告)号：US20160068851A1

公开(公告)日：2016-03-10

申请号：US14844731

申请日：2015-09-03

Applicant: Dow AgroSciences LLC

Inventor： Manju GUPTA ,  Sara BENNETT ,  Sandeep KUMAR ,  Donald MERLO ,  Nagesh SARDESAI

IPC: C12N15/82 ,  C12N15/74

CPC classification number: C12N15/8205 ,  C12N15/743

Abstract: The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.

Abstract translation: 本公开提供了用于生产和使用相对于亲本菌株缺乏RecA活性的根癌土壤杆菌菌株（例如LBA4404）的新型组合物和方法。 还公开了与根癌土壤杆菌的其他基因缺陷型菌株的组合。 具体地，提供了两个示例性的recA减毒株，UIA777，其中氯霉素抗性基因破坏recA基因和UIA770，其中卡那霉素抗性基因破坏recA基因。

公开(公告)号：US20150101083A1

公开(公告)日：2015-04-09

申请号：US14505055

申请日：2014-10-02

Applicant: Dow AgroSciences LLC

Inventor： Manju GUPTA ,  Sandeep KUMAR ,  Navin ELANGO ,  Karthik Narayna MUTHURAMAN ,  Jeffrey BERINGER ,  Huixia WU ,  Nagesh SARDESAI

IPC: C12N15/82

CPC classification number: C12N15/8227 ,  C12N15/8216 ,  C12N15/8222

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays metallothionein-like gene regulatory elements.

Abstract translation: 提供的是使用玉米金属硫蛋白样基因调控元件在植物细胞和/或植物组织中表达转基因的构建体和方法。